LB-Cm Agar plates - I know what LB is, what is Cm? (Sep/28/2009 )

Hello,

Just reading a paper where a library of transformed cells are plated out onto LB-Cm plates.
I know that LB stands for Luria Broth, but what is Cm???

If anyone can help de-mystify this, that would be appreciated.

Thank you in advance

LB

-Luria Bertani-

Luria Bertani on Sep 28 2009, 09:15 PM said:

Chloramphenicol

-fishdoc-

Luria Bertani on Sep 28 2009, 10:15 PM said:

I know that LB stands for Luria Broth...

My first paper on lysogeny (5), describing the modified single-burst experiment and the isolation of P1, P2, and P3, also contained the formula of the LB medium which I had concocted in order to optimize Shigella growth and plaque formation. Its use has since become very popular. The acronym has been variously interpreted, perhaps flatteringly, but incorrectly, as Luria broth, Lennox broth, or Luria-Bertani medium. For the historical record, the abbreviation LB was intended to stand for "lysogeny broth."

Bertani, G. 2004. Lysogeny at mid-twentieth century: P1, P2, and other experimental systems. J Bacteriol 186(3):595-600

-HomeBrew-

Hi there,

I have made up some stocks of Chloramphenicol in Ethanol (since it is rather insoluble in water).
I was just wondering if it stores well at -20 degrees C? And how stable it is in agar plates that are refrigerated at 4 degrees C?

I know that my Kanamycin plates can still be used after 2-3 months if refrigerated- the antibiotic is still active then.

-Luria Bertani-

Normally, we do store them not more than 30 days.
Back in the 70ies someone did assay the stability of different antibiotics on agar plates.
Here is the link!

Regards,
p

-pDNA-

Thank you, this is very helpful!

-Luria Bertani-