how to know a sequene for a specific gene & produce it - (Sep/27/2009 )

hello ev 1

we have a human gene of 500 bp which is responsible for production of a specific antibody for a certain infection

we need
1. to know its sequence
2. produce it commercially as a pure human antibody to use it as treatment for this infection .

what are the techniques i have to use to achieve this goals .
in detail plz as much as u can


many thanks for every 1 thinking to help me.

D lofi

Give me 25% share in your product and I'll be glad to help... ;-0

If you know the gene, you can find the sequence through Pubmed. Then you need to make primers and clone the gene (from mRNA, I suspect). Let me know when you've done that much...

My bid is 10%

K BUT U SHOULD BE SURE OF UR ANSWER 1ST BUDDY SWANNY

THE PROBLEM IS THAT I DONT KNOW ANY THING ABT THIS GENE
SO HOW CAN I KNOW THE SEQUENCE??????????????
30% IF U CAN HELP ME B THIS LOOOOOOOOOOOOOL


I DON NEED UR DISCOUNT CAUSE I NEED DA ANSWER BEFORE THE NEGOTIATIONS GLEB KUDR.

Hey LOFI,
First of all, please stop shouting at us (ie take the caps lock off...).

Second, your initial post said that you "had" a gene. That kind of suggests you know something about it. If you don't want to totally publicise the gene, send me a PM and we can talk further.

Human genome are sequenced than you have a gene> you know its sequence.

Than design primers and PCR it (from RNA template I suppose)

Than cloning into simple vector and sequencing to be sure it's target gene and not broken clone.

Than cut&paste into transmissible/production vector

..(transformation, obtaining stable protein accumulation and so on)..

Profit!