DMMB dye for GAG measurement in chondrogenesis - Is this method still being used? (Sep/15/2009 )

hi everyone,

i need a bit of help
i have been trying to track the level of chondrogenesis by measurement of GAG level
in the cells. i`ve read about the DMMB method, but.... but.... i found it hard
to get more recent information regarding the DMMB method,
all the things i have read seem to be old, i`d like to ask if anyone
know about the DMMB method? is it still being performed?

if i use a layer of GAG coating upon the culture dish, can i add DMMB into the medium to detect the GAG level?
can the GAG in the cells be secreted and released into the medium?

pls--- thnks a lot!!

yue

tzyy yue on Sep 16 2009, 02:00 AM said:

hi everyone,

i need a bit of help
i have been trying to track the level of chondrogenesis by measurement of GAG level
in the cells. i`ve read about the DMMB method, but.... but.... i found it hard
to get more recent information regarding the DMMB method,
all the things i have read seem to be old, i`d like to ask if anyone
know about the DMMB method? is it still being performed?

if i use a layer of GAG coating upon the culture dish, can i add DMMB into the medium to detect the GAG level?
can the GAG in the cells be secreted and released into the medium?

pls--- thnks a lot!!

yue