assay for penicillinase - (Sep/09/2009 )
Hello everybody,
Does anyone know how to perform assay for penicillinase?. I ve heard of an assay with nitroefin, but this reagent is very expensive. I have read about spectrophotometric assays using a UV spectrophotometer.Pls help
-chn09-
chn09 on Sep 9 2009, 12:39 PM said:
Hello everybody,
Does anyone know how to perform assay for penicillinase?. I ve heard of an assay with nitroefin, but this reagent is very expensive. I have read about spectrophotometric assays using a UV spectrophotometer.Pls help
Does anyone know how to perform assay for penicillinase?. I ve heard of an assay with nitroefin, but this reagent is very expensive. I have read about spectrophotometric assays using a UV spectrophotometer.Pls help
Have a look at these articles:
Yamabe S. Rapid spectrophotometric method for the determination of beta-lactamase activity. J Antimicrob Chemother. 1980;6:261-6.
Waley SG. A spectrophotometric assay of b-lactamase activity on penicillins. Biochem J. 1974;139:789-9.
Hope this helps
-klinmed-