Immunocytochemistry - (Sep/08/2009 )

Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before ‎adding the antibody and keep the cells after fixation with 4% PFA?‎
Thanks ‎

-Postdoc_G-

Postdoc_G on Sep 8 2009, 02:53 PM said:

Hi, I am planning to do Immunocytochemistry to cells. Is it possible to stop before ‎adding the antibody and keep the cells after fixation with 4% PFA?‎
Thanks ‎

Yes, I keep them in 1x PBS at 4°C until ready for staining.

-Dr Teeth-

Don't keep them for too long though, a week or two maximum.

-bob1-

And don't let them dry out!!!!

-miBunny-