antibody crossreactivity - (Sep/04/2009 )

hi all

can we use human anti C3 (complement protein) antibody against mouse C3? i have read that human C3 and mouse C3 have 77% homology.

ujla80 on Sep 4 2009, 06:57 AM said:

Is your antibody monoclonal or polyclonal? If it is monoclonal it will only cross-react if the region to which the human antibody was raised is identical in the mouse protein. If it's a commercial antibody then the product details will tell you with which species it cross-reacts,

P