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Outer membrane preparation protocol. - (Sep/02/2009 )

Hi all,
I want to extract the outer membrane form Ecoli. I am looking for a protocol.

If anyone has the basic protocol for outer membrane extraction ,please give me.

-Volga-

What you try to get the membrane for? I have come across come articles on membrane breaking using probe sonicator but I not sure whether it suits your needs.

-adrian kohsf-