2005 )

The A260/A280 ratio of my RNA purification is often >2 sometimes even >3. Does anyone know the cause of this and how to ommit it?

The RNA is purifyied from barley plants with the RNAeasy kit from qiagen with an on-collum DNase treatment. The RNA is going to be used for real time RT-PCR. Is the high ratio a problem for this application?

-MarianneB-

hi
there were discussions
""../../forums/inde...?showtopic=7142
and also
""../../forums/inde...?showtopic=5968
and finally part of discussion
""../../forums/inde...?showtopic=6142
Fred

-fred_33-

Thanks, I'll try and see if there are a good idea in these discussions

-MarianneB-

I've solved the problem. It seems that reducing the amount of starting material does the trick. The ratio is now 1,9 without any decline in yield.

-MarianneB-