transfection efficiency - mammalian cell transfections (Jul/12/2004 )

i want to calculate transfection efficiency.

the expt is: i plated 10(6) cells, used calcium phosphate method, transfected GFP plasmid. after 7 days on G418 selection, how do i calculate the efficiency of transfection based on amount of DNA used and the cell number plated ?
plz sueegst me any book or weblink which will give me a n answer.

Hi ,aditya


I have abtained one student sheet for calculating tansfection efficiency and hope it help you.Would you like gave me your E-mail address?

weimin42