Maximum solubility of genomic DNA - (Aug/28/2007 )

Hi

I'm trying to concentrate gDNA above 50 ug/uL. I'm wondering if it is possible.
Now, I'm evaporating the solvent (10 mM Tris, pH 8) using a centrifuge with a vacuum equipment at 65 C. After the concentrating procedure I'd like to hybridize gDNA with a probe having a fluorophore.

Is there any good method?

Thank you for reading my post.

Add salt, two volumes of EtOH, which will precipitate the DNA. Collect DNA, wash in 70% a few times. Dry but not to completion (1/2 hr on bench) and add enough TE to get the concentration you require.

This works well if you have enough DNA.