primer sets to do pyrosequencing - (Jul/02/2007 )
Prior to using methyleasy kit, I used Zymoresearch of EZ methylaytion kit.  
After changing kit, I have been able to identify PCR band.  thank you~!!!
but now, I have had  dfficult problems. I am going to do pyrosequencing to identify methylation status.  and I made primer sets through PSQ  program.  Firstly, Have you ever done pyrosequencing?  If you didn't  have  experience,  I'm affraid...ㅠ.ㅠ...
Some primer sets is good. others is too bad I can't identify PCR band. 
I wonder.....................
How can I identify problems of primer sets?? or How can I make possible primer set efficiently to do  pyrosequencing? 
please help me~~!!
I have no done pyrosequencing myself but I heard that the folks at biotage are very good at solving problems with your primers...
I wonder.~~  first of all. thanks~~@!! 
 I didn't understand your explain well .   Is your mean that biotage is able to help to make PCR primer sets to do pyrosequencing? 
 Excuse me~!!~ I need more explain in detail.
Biotage support can provide you with more information and as far as I know they can also help you with the primer design.
