PEG to seperate Plasmide from Protein? - (Jul/14/2006 )
I was told you can use PEG tp precipitate protein when you have a plasmide purification that contains a bigger protein contamination (precipitating the DNA yields a pellet almost impossible to resolve and there is a bad 260/280nm ratio for the solution).
Is that true?
I only found informations of how to precipitate DNA from plasmide preps with PEG8000 and NaCl. But I would like to precipitate the protein inside and than just do a Isoprop precipitation of the remaining plasmide.
Anyone has a protocol or hint how to do that if its possible at all?
-wincel-
a cleaner method is to use phenol chloroform iaa...  
isn't it feasible?
-fred_33-
QUOTE (fred_33 @ Jul 19 2006, 07:07 PM) 
a cleaner method is to use phenol chloroform iaa... ![huh.gif]() 
 
isn't it feasible?
isn't it feasible?
It would be, if we did not have to prevent precipitation as the pellet isn't solving anymore and we have a maxi prep with 10,5ml of Eluate - this would need tons of Chlo/Phe/etc. I hoped PEG is possible with smaller Volumes?
-wincel-
