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Protocols
- Complete Guide to Flow Cytometry (FACS) for Cell Cycle
(Andrew Burgess, PhD)
A complete Guide to Cell Cycle FACS, detailing how to fix, stain, aquire and analyze cells using a BD FACSCalibur and CellQuest software.
http://sciencetechblog.files.wordpress.com/2011/05...
Added: Wed May 25 2011, Hits: 4972, Reviews: 0 Write review - Complete Guide to Flow Cytometry for Cell Cycle (Andrew Burgess, PhD)
A complete Guide detailing how to fix, stain, aquire and analyze cells using a BD FACSCalibur and CellQuest software
http://www.sciencetechblog.com/ScienceTechBlog/Sci...
Added: Thu Jun 10 2010, Hits: 12366, Reviews: 0 Write review - FACS Troubleshootings (Protocol Online)
Archives of FACS troubleshootings
""../../../biology-forums/flow...
Added: Wed Feb 11 2009, Hits: 3756, Reviews: 0 Write review Cached - Flow Cytometry Analysis (Springer Lab, Harvard University)
Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules.
http://www.cbrinstitute.org/labs/springer/protocol...
Added: Wed Aug 14 2002, Hits: 5615, Reviews: 0 Write review Cached -
Advanced Methodologies in Flow Cytometry
(Cytomety Lab, Purdue Univ)
A large collection of methodological paper on cytometry
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 3682, Reviews: 0 Write review Cached -
Direct Immunofluorescence Staining
(U of Florida Flow Cytometry Core Laboratory)
This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling.
http://www.biotech.ufl.edu/FlowCytometry/direct.ht...
Added: Tue May 14 2002, Hits: 1339, Reviews: 0 Write review Cached -
Flow Cytometry: Indirect Immunofluorescence Staining of Human Platelets
(BD Pharmingen)
Protocol for general use.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Sat Jul 27 2002, Hits: 2178, Reviews: 0 Write review Cached -
Fluorescein Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/flabel.html
Added: Tue May 14 2002, Hits: 1818, Reviews: 0 Write review Cached -
Fluorescence and Flow Cytometry Tutorials
(Invitrogen )
These tutorials provide a great overview of the basics of fluorescence and flow cytometry, covering the theory and instrumentation and are a great resource for new students or researchers.
http://www.invitrogen.com/site/us/en/home/support/...
Added: Tue May 18 2010, Hits: 2053, Reviews: 0 Write review -
Immunofluorescent Staining for FACS Analysis
(Flow Cytometry Core Facility, PrincetonUniversity)
General immunofluorescence staining protocol using directly and indirectly conjugated antibodies
http://www.molbio1.princeton.edu/facility/flowcyt/...
Added: Sat Feb 21 2009, Hits: 2613, Reviews: 0 Write review Cached -
Phycoerythrin Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/pelabel.html
Added: Tue May 14 2002, Hits: 1288, Reviews: 0 Write review Cached -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Gives detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 2274, Reviews: 0 Write review Cached -
Staining Procedure for Flow Cytometric Detection of Human Cyclins
(BD PharMingen)
This is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a good starting point. However, investigators may need to optimize protocols for their own experimental system. It is particularly useful to refer to the published literature regarding protocols typically used for a given type of protein.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 1654, Reviews: 0 Write review Cached -
Standard Flow Cytometry (FACS) Protocol
(Jackson Lab)
FACS Analysis Using Peripheral Blood Cells
http://www.jax.org/imr/facs.html
Added: Tue May 14 2002, Hits: 8449, Reviews: 0 Write review Cached
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