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Protocols
- Cell Cytotoxicity Assay (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatments). Large amounts of label is released into the supernatant upon disruption of membrane integrity by the killing process and can be measured in a gamma-counter.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
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3H Thymidine-Incorporation Assay for Rat-1a cells
(iProtocol)
http://iprotocol.mit.edu/protocol/128.htm
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3H-Thymidine Uptake by Cultured Cells
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
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3H-Thymidine Uptake by Cultured Cells
(iProtocol)
A radioactive method for measuring cultured cell growth with 3H-thymidine.
http://iprotocol.mit.edu/protocol/56.htm
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96-Well Cell Proliferation Assay
(Promega)
Colorimetric method for determining the number of viable cells in proliferation or chemosensitivity assays.
http://www.promega.com/tbs/tb169/tb169.html#iv
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Cell Proliferation Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Cell proliferation assay by cell counting.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
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Cell Viability Assay
(http://www.hallym.ac.kr/~gene)
Dye exclusion method
http://www.hallym.ac.kr/~gene/MoleGene/protocol/ki...
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Cr Release Cytotoxicity assay
(The Science Advisory Board)
Cytotoxic activities of mNK cells are examined in 51Cr release assay from target cells
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 1033, Reviews: 0 Write review Cached -
Crystal Violet Assay
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay, crystal violet, stains DNA. Upon solubilization, the amount of dye taken up by the monolayer can be quantitated in a spectrophotometer or plate reader.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Mon Oct 28 2002, Hits: 961, Reviews: 0 Write review Cached -
Cytotoxicity Assays Protocol
(Hedrick Lab, UCSD Cancer Center)
Cell-mediated cytotoxicity is determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted and resuspended in 100 mCi of Na51Cr...
http://hedricklab.ucsd.edu/Protocol/cyto.html
Added: Tue Dec 10 2002, Hits: 2796, Reviews: 0 Write review Cached -
Detection of BrdU Incorportation in DNA Synthesizing Cells
(BD PharMingen)
A standard protocol for detecting DNA in cells.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 2342, Reviews: 0 Write review Cached -
Determination of IC50
(The Science Advisory Board)
In vitro whole cell assay by [H3] Hypoxanthine uptake assay.
http://www.scienceboard.net/resources/protocols.as...
Added: Wed Apr 21 2004, Hits: 1867, Reviews: 0 Write review Cached -
Methods for Cell Death Staining
(Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
Includes trypan blue staining, propidium iodide staining of living cells and fixed cells, Hoescht staining.
http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
Added: Tue Oct 01 2002, Hits: 1747, Reviews: 0 Write review Cached -
Proliferation Assay-[3H] Thymidine incorporation
(Hannun/Obeid Lab)
http://www.musc.edu/bcmb/ceramide/protocols/0017.h...
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Timing of Cycles
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 1179, Reviews: 0 Write review Cached
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