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LB medium - eLabJournal.com
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Category: Media
Author: Admin eLabJournal

Labels: E. coli

Materials
 

Tryptone
Yeast extract

NaCl
58.44 g mol-1

Experiment settings

Volume of LB medium: L 

Step 1
Prepare L  of LB medium by adding:
  • g  Tryptone
  • g  Yeast extract
  • g  NaCl
Step 2
Add dH2O until the total volume of
Step 3
Set to pH 7 by adding one or two drops of NaOH
Step 4
Make aliquots and autoclave