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Compatible Cohesive Ends  
New England Biolabs Technical Literature - Updated  restriction_sm.gif (1260 bytes)
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Compatible Cohesive Ends

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Restriction endonucleases that produce compatible cohesive ends often produce recleavable ligation products. The combinations listed were identified by computer analysis, and although we have tried to ensure their accuracy, they have not necessarily been confirmed by experimentation.

Where isoschizomers exist, only one member of each set is listed. Only enzymes available from New England Biolabs have been listed.

Enzymes that have degenerate recognition sequences (i.e. recognize more than one sequence) are followed by a specific sequence in parentheses and are only listed if a non-degenerate equivalent does not exist. Be aware that these degenerate enzymes will cleave sequences in addition to the one listed.

A "--" denotes a ligation product that cannot be recleaved.

|  A  |  B  |  C  |  D  |  E  |  H  |  K  |  M  |  N  |  P  |  R  |  S  |  T  |  X  |
 

Enzyme Ligated To Recleaved By
Acc65 I
(G/GTACC)		 Ban I (G/GTACC) Acc65 I, Ban I, Kpn I, Nla IV, Rsa I 
BsiW I, BsrG I Rsa I
Acc I
(GT/CGAC) Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I --
(GT/CGAC) Cla I, BstB I, Taq I Taq I
Aci I
(C/CGC) 		Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I --
BsaH I (GR/CGCC), HinP1 I, Nar I Aci I
Hpa II Hpa II
Acl I
(AA/CGTT)		 Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq I --
Afl III
(A/CGCGT) Asc I, BssH II BstU I, Hha I
(A/CATGT) BspH I, Nco I, Pci I Nla III
(A/CGCGT) Mlu I Afl III, BstU I, Mlu I
Age I
(A/CCGGT)		 Ava I (C/CCGGG), Xma I Hpa II, Nci I, ScrF I
BsaW I, BspE I BsaW I, Hpa II
BsrF I (A/CCGGT), SgrA I (CA/CCGGTG) Age I, BsaW I, BsrF I, Hpa II
NgoM IV BsrF I, Hpa II
Apa I
(GGGCC/C)		 Ban II (GGGCC/C), Bsp1286 I (GGGCC/C) Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 I
ApaL I
(G/TGCAC)		 Sfc I (C/TGCAG) Bsg I
Apo I
(A/AATTY) EcoR I Apo I, Tsp509 I
(G/AATTY) EcoR I Apo I, EcoR I, Tsp509 I
(R/AATTY) Mfe I, Tsp509 I Tsp509 I
Asc I
(GG/CGCGCC)		 Afl III (A/CGCGT), Mlu I BstU I, Hha I
BssH II BssH II, BstU I, Cac8 I, Hha I
Ase I
(AT/TAAT)		 Bfa I, Csp6 I, Nde I --
Mse I Mse I
AsiS I
(GCGAT/CGC) 		BsiE I (CGAT/CG) Dpn II, Pvu I
Pac I Mse I
Pvu I Dpn II, Pvu I
Ava I
(C/CCGGG) Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG) Hpa II, Nci I, ScrF I
(C/TCGAG) Xho I Ava I, Taq I, Xho I
(C/TCGAG) Sal I Taq I
(C/CCGGG) Xma I Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I
Ava II
(G/GWCC)		 PpuM I (RG/GACCY) Ava II, Nla IV, Sau96 I
Rsr II Ava II, Sau96 I
PpuM I (RG/GTCCY) Ava II, BsmF I, Nla IV, Sau96 I
Avr II
(C/CTAGG)		 Nhe I, Spe I, Xba I Bfa I
Sty I (C/CTAGG) Avr II, Bfa I, BsaJ I, Sty I
BamH I
(G/GATCC)		 Bcl I, Dpn II Alw I, Dpn II
Bgl II, BstY I (R/GATCY) Alw I, BstY I, Dpn II
BstY I (G/GATCC) Alw I, BamH I, BstY I, Dpn II, Nla IV
Ban I
(G/GTACC) Acc65 I Acc65 I, Ban I, Kpn I, Nla IV, Rsa I
(G/GCGCC) Kas I Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV
(G/GTACC) BsiW I, BsrG I Rsa I
Ban II
(GGGCC/C) Apa I, Bsp1286 I (GGGCC/C) Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I
(GAGCT/C) Bsp1286 I (GAGCT/C), Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
Bcl I
(T/GATCA) 		BamH I, BstY I (R/GATCY) Alw I, Dpn II
Bgl II, Mbo II Dpn II
Bfa I
(C/TAG)		 Ase I, Csp6 I, Mse I, Nde I --
Bgl II
(A/GATCT)		 BamH I, BstY I (R/GATCY) Alw I, BstY I, Dpn II
Bcl I, Dpn II Dpn II
BsaH I
(GR/CGYC) Acc I (GT/CGAC), Cla I, BstB I, Taq I --
(GA/CGYC) Aci I, HinP1 I Hga I
(GG/CGYC) Aci I, HinP1 I Hha I
(GG/CGYC) Hpa II Aci I
(GA/CGYC) Nar I BsaH I, Hga I
(GG/CGYC) Nar I Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV
BsaW I
(W/CCGGW)		 Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG) Age I, BsaW I, BsrF I, Hpa II
Ava I (C/CCGGG), Xma I Hpa II, Nci I, ScrF I
BspE I BsaW I, BspE I, Hpa II
BsrF I (R/CCGGY), NgoM IV BsrF I, Hpa II
NgoM IV Hpa II
BsiE I
(CGAT/CG) Pac I Mse I
(CGAT/CG) Pvu I BsiE I, Dpn II, Pvu I
(CGGC/CG) Sac II Aci I
BsiHKA I
(GTGCA/C) 		Bsp1286 I (GTGCA/C) BsiHKA I, Bsp1286 I
Bsp1286 I (GAGCA/C) BsiHKA I, Bsp1286 I
Bsp1286 I (GAGCT/C), Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
Nsi I --
Pst I, Sbf I Bsg I
BsiW I
(C/GTACG)		 Acc65 I, Ban I (G/GTACC), BsrG I Rsa I
Bsp1286 I
(GGGCC/C) Apa I, Ban II (GGGCC/C) Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I
(GTGCA/C) BsiHKA I ApaL I, BsiHKA I, Bsp1286 I
(GGGCC/C) Ban II (GGGCC/C) Ban II, Bsp1286 I
(GAGCT/C) Ban II (GAGCT/C), BsiHKA I, Sac I Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I
(GWGCW/C) BsiHKA I BsiHKA I, Bsp1286 I
(GTGCA/C) Nsi I --
(GTGCA/C) Pst I, Sbf I Bsg I
BspE I
(T/CCGGA)		 Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG) BsaW I, Hpa II
Ava I (C/CCGGG), Xma I Hpa II, Nci I, ScrF I
BsaW I BsaW I, BspE I, Hpa II
BsrF I (R/CCGGY), NgoM IV Hpa II
BspH I
(T/CATGA)		 Afl III (A/CATGT), Nco I, Sty I (C/CATGG) Nla III
BsrF I
(A/CCGGY) Age I, BsaW I Age