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Recleavable Blunt Ends  
New England Biolabs Technical Literature - Updated  restriction_sm.gif (1260 bytes)
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Recleavable Blunt Ends

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The table below lists combinations of blunt-end restriction endonucleases that produce recleavable ligation products.The combinations listed were identified by computer analysis, and although we have tried to ensure their accuracy, they have not necessarily been confirmed by experimentation.

Enzymes that have degenerate recognition sequences (i.e. recognize more than one sequence) are followed by a specific sequence in parentheses and are only listed if a non-degenerate equivalent does not exist. Be aware that these degenerate enzymes will cleave sequences in addition to the one listed. Where isoschizomers exist, only one member of each set is listed. Only commercially available enzymes have been listed

|  A   |  B  |  D  |  E  |  F  |  H  |  M  |  N  |  P  |  R  |  S  |

Enzyme Ligated to Recleaved by
Afe I
(AGC/GCT)		 BsrB I, MspA1 I (CMG/CGG) Hpa II
BstZ17 I Alu I
EcoR V SfaN I
Sfo I Hae II, Hha I
Fsp I Hha I
Nae I Aci I, Fnu4H I
Sma I Aci I
Alu I
(AG/CT)		 BsrB I, MspA1 I (CMG/CGG) Aci I
BstZ17 I Rsa I
MspA1 I (CMG/CTG), Pvu II Alu I
EcoR V Mbo I
Sfo I Hae III
 BmgB I
(CAC/GTC) 		Pml I Pml I
Aat II Aat II
BsaA I    
(YAC/GTR) BsrB I, MspA1 I (CMG/CGG) Hpa II
(CAC/GTR) Pml I BsaA I, Pml I
(TAC/GTR) Pml I, SnaB I BsaA I
(TAC/GTR) SnaB I BsaA I, SnaB I
BsrB I
(GAG/CGG)		 Alu I, MspA1 I (CMG/CTG), Pvu II Alu I
BsaA I (YAC/GTR), Fsp I, Hinc II (GTY/GAC), Nae I, Pml I, Sma I, SnaB I Mnl I
BstZ17 I Rsa I
Afe I Mnl I
EcoR V Mbo I
Sfo I Hae III, Mnl I
MspA1 I (CMG/CGG) Aci I, BsrB I
Rsa I, Sca I BsmA I
BstU I
(CG/CG)		 BsrB I, MspA1 I (CMG/CGG) Aci I, BstU I
BstZ17 I Rsa I
EcoR V Mbo I
Sfo I Hae III
Nru I BstU I
BstZ17 I
(GTA/TAC)		 Alu I, BsrB I, BstU I, Hae III, Msc I, MspA1 I(CMG/CKG), Nru I, Pvu II, Stu I Rsa I
Afe I Alu I
Hinc II (GTY/GAC) Acc I
Ssp I Tsp509 I
Dra I
(TTT/AAA)		 Hinc II (GTY/AAC), Hpa I Mse I
Nru I Taq I
Pme I, Swa I Dra I, Mse I
EcoR V
(GAT/ATC)		 Alu I, BsrB I, BstU I, MspA1 I (CMG/CKG), Pvu II Mbo I
Hae III, Msc I, Stu I Alw I, Mbo I
Afe I, Sfo I, Fsp I SfaN I
Nru I Mbo I, Taq I
Fsp I
(TGC/GCA)		 BsrB I, MspA1 I (CMG/CGG) Hpa II
Afe I, Sfo I Hha I
EcoR V SfaN I
Nae I Aci I, Fnu4H I
Sma I Aci I
Hae III
(GG/CC)		 BsrB I, MspA1 I (CMG/CGG) Aci I
BstZ17 I Rsa I
Msc I, Stu I Hae III
EcoR V Alw I, Mbo I
Sfo I Hae III, Asu96 I
Hinc II (GTY/GAC) BsmF I
Hpa I
(GTT/AAC)		 Dra I, Pme I Mse I
Hinc II (GTY/AAC) Hinc II, Hpa I, Mse I
Hinc II (GTY/GAC) Hinc II
Nru I Taq I
Hinc II  
(GTC/RAC) BsrB I, MspA1 I (CMG/CGG) Hpa II
(GTC/RAC) BstZ17 I Acc I
(GTC/RAC) Hae III, Msc I, Stu I BsmF I
(GTT/RAC) Dra I, Pme I Mse I
(GTC/RAC) Hpa I Hinc II
(GTT/RAC) Hpa I Hinc II, Hpa I, Mse I
(GTT/RAC) Nru I Taq I
(GTC/RAC) Rsa I, Sca I Tsp45 I
Msc I
(TGG/CCA)		 BsrB I, MspA1 I (CMG/CGG) Aci I
BstZ17 I Rsa I
Hae III, Stu I Hae III
EcoR V Alw I, Mbo I
Sfo I Hae III, Sau96 I
Hinc II (GTY/GAC) BsmF I
MspA1 I  
(CAG/CKG) Alu I Alu I
(CCG/CKG) Alu I, Hae III, Msc I, Stu I Aci I
(CCG/CKG) BsaA I, Fsp I, Hinc II (GTY/GAC), Pml I, SnaB I Hpa II
(CAG/CKG) BsrB I, Pvu II Aci I, MspA1 I
(CCG/CKG) BsrB I Aci I, BsaJ I, BstU I, MspA1 I, Sac II
(CMG/CKG) BstZ17 I Rsa I
(CCG/CKG) BstU I, Nru I Aci I, BstU I
(CCG/CKG) Afe I Hpa II