PCR clean up using 3M sodium acetate and chilled absolute ethanol

1. For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.
   • 2 ul of 3M sodium acetate (NaOAc) pH 4.5
   • 40 ul chilled absolute ethanol.
2. Transfer the PCR product (20ul) into the tube containing the mixture.
3. Vortex the tube and then store it at -20 for 30-50 min.
4. Spin for 30 min at maximum speed 14.000 rpm.
5. Rremove the supernatant carefully and don't disturb the pellet. ( Do not touch the bottom of the tube)
6. Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min.
7. Remove the supernatant and then dry the pellet using vacuum centrifuge. (do not apply heat)
8. Resuspend the pellet in 30 ul in sterile water.