ELISA experiment - High background (Dec/12/2005 )
Hi everybody these days I'm doing in-house ELISA and I was getting very nice results, and suddenly for the last 2 days I'm getting very high background which means the whole plate is giving the same high yallow color including the control negative and positive,please help me
-microbiologydepartment-
QUOTE (microbiologydepartment @ Dec 12 2005, 11:29 AM)
Hi everybody these days I'm doing in-house ELISA and I was getting very nice results, and suddenly for the last 2 days I'm getting very high background which means the whole plate is giving the same high yallow color including the control negative and positive,please help me ![sad.gif]()
Did you change anything in your assay ? Antibodies ? Solution ? Elisa washer ?
Pesji
-pesji-