Protocols
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PEG Purification of PCR Products
(GSCWU)
This protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing
http://genome.wustl.edu/tools/protocols/index.php?...
Added: Tue May 14 2002, Hits: 169, Reviews: 0
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Purification and Sequencing of PCR Product
(Donis-Keller lab)
This method is used to sequence directly a specific PCR product after large scale amplification. The reaction should be carried out in the usual fashion, except that after optimization of annealing temperature and other conditions in the 5 µl volume, a large scale 50 µl (10X) total reaction volume should be used to generate sufficient PCR product for sequencing.
http://hdklab.wustl.edu/lab_manual/pcr/pcr3.html
Added: Tue May 14 2002, Hits: 363, Reviews: 0
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