Protocols
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Chemical Sequencing of DNA
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
This is a rapid method for chemical DNA sequencing which is commonly used as ladder for footprinting reactions or for sequencing of short DNA oligonucleotides.
http://www.fhcrc.org/labs/hahn/methods/mol_bio_met...
Added: Sat Mar 27 2004, Hits: 108, Reviews: 0
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Maxam-Gilbert Sequencing
(The Morimoto Lab, Northwestern University)
A modified, abbreviated version of the Maxam-Gilbert sequencing is described.
http://www.biochem.northwestern.edu/ibis/morimoto/...
Added: Sat Mar 27 2004, Hits: 118, Reviews: 0
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- Preparation of G+A Marker ( Protocol Online)
Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing reaction and works fine.
Added: Tue May 14 2002, Reviews: 0
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