Protocols
- Indirect ELISA
(Abcam)
For accurate quantitative results, always compare signal of unknown samples against those of a standard curve. Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy.
http://www.abcam.com/index.html?pageconfig=resourc...
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Indirect ELISA
(Kitto Lab, The University of Texas at Austin)
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
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Indirect ELISA
(Sigma)
http://www.sigmaaldrich.com/life-science/cell-biol...
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