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- Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
The author has made a search for an alternate nutrient (serum supplement) source for lymphocyte cultures. This report describes the procedure for separation of serum from adult cattle and its successful use, which gives an excellent mitotic index for chromosomes investigations. This procedure can be applied practically in all the laboratories requiring serum, particularly in the countries with limitations on cow slaughtering. In this process there is no need of extra care post collection and animals can be used for about a year. Then new animals are examined and selected, these can act as healthy donors like in humans. Supplementation with adult cattle serum has been found to give desired mitotic division of lymphocytes of several animal species for chromosome evaluation.
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Chromosome Conformation Capture (3C)
(Epigenome Network of Excellence)
The 3C (Chromosome Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus.
http://www.epigenome-noe.net/researchtools/protoco...
Added: Wed May 27 2009, Hits: 201, Reviews: 0 Write review Cached -
Chromosome Conformation Capture (3C) (alternate technique)
(Epigenome Network of Excellence)
This is an alternative 3C protocol for 3C which produces a greater yield of 3C material; useful if the 3C material is going to be processed further.
http://www.epigenome-noe.net/researchtools/protoco...
Added: Wed May 27 2009, Hits: 188, Reviews: 0 Write review Cached -
FISH on Human Chromosomes and Interfase Nuclei
(Institute of Pathology,Humboldt-University of Berlin)
Gives details on slide preparation, plasmid, cosmid and YAC library probe preparation and detection
http://allserv.rug.ac.be/~fspelema/neubla/protocol...
Added: Tue May 14 2002, Hits: 1444, Reviews: 0 Write review Cached -
Giemsa-trypsin Banding
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2455.2...
Added: Sat Oct 19 2002, Hits: 1320, Reviews: 0 Write review -
Human Chromosome Preparation
(Doug Lundberg)
A blood sample is taken and white blood cells grown in a special medium for three days under the influence of the mitotic stimulant..
http://academy.d20.co.edu/kadets/lundberg/chromoso...
Added: Tue May 14 2002, Hits: 276, Reviews: 0 Write review Cached