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Ambion's Tips From the Bench: RNA Conversions

Technical Resources > The RNA Files > Tips from the Bench

Tips from the Bench

RNA Conversions

When isolating RNA, it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also beneficial to know rough expression levels for rare to moderately abundant transcripts when performing Northern analysis or RPAs, e.g. in order to set up a meaningful standard curve for absolute quantitation, or to know how long of an exposure is reasonable. These numbers provide a point of reference against which experimental procedures and results can be checked. Tables 1 and 2 provide general guidelines for estimating RNA yields from a variety of cells and tissues. Remember that RNA yields from analogous tissues in different organisms can also vary:

Ordering Information
For prices and availability, please contact Customer Service.

Cat#	Product Name	Size
1356	RT-PCR Competitor Construction Kit	10 rxns
1710	RETROscript^® Kit	40 rxns
1716	QuantumRNA™ Classic 18S Internal Standard	100 rxns
1717	QuantumRNA™ Classic II 18S Internal Standard	100 rxns
1718	QuantumRNA™ Universal 18S Internal Standard	100 rxns
1720	QuantumRNA™ Beta-actin Internal Standards	100 rxns
1902	Paraffin Block RNA Isolation Kit	100 rxns
1910	ToTALLY RNA™ Kit	10 g tissue
1911	RNAqueous®-Midi Kit	15 purifications
1912	RNAqueous® Kit	50 purifications
1913	RNAqueous®-Blood Module	50 purifications
1914	RNAqueous®-4PCR Kit	30 rxns
1915	Poly(A)Pure™ Kit	6 rxns
1918	MicroPoly(A)Pure™ Kit	20 rxns
1920	RNAqueous®-96 Kit	192 purifications
2050	SuperTaq™ Polymerase (Cloned) 5 U/µl	50 U
2052	SuperTaq™ Polymerase (Cloned) 5 U/µl	250 U
2054	SuperTaq™ Plus Polymerase (Cloned) 5 U/µl	50 U
2056	SuperTaq™ Plus Polymerase (Cloned) 5 U/µl	250 U
9690	Plant RNA Isolation Aid	10 ml
9736	RNAwiz™	100 ml
9890	DNAZap™	250 ml of each solution
10035	96-well PCR Plate w/ 8 Strip Caps	10 plates
10036G	96-well PCR Plate Replacement Caps	125 strips
1906	DNA-free™	50 rxns
10065	Spin Columns and Tubes	100 units
12225	Thin-walled, Frosted Lid, RNase-free PCR Tubes	1000 x 0.2 ml
12230	8-strip PCR Tubes & Caps, 0.2 ml	125 strips (1000 tubes)
12250	Thin-walled, Dome Cap, RNase-free PCR Tubes	1000 x 0.5 ml
12275	Thin-walled, Frosted Lid, RNase-free PCR Tubes	1000 x 0.5 ml