In situ hybridization of genes and mRNA is most often based on polynucleotide probes. However, the specificity of polynucleotide probes has not been thoroughly investigated and a rational probe design concept was still missing, because the well established concept for oligonucleotide probe design cannot be transferred to polynucleotides.

We recently developed a concept and software (PolyPro) for rational design of polynucleotide probe mixes to identify particular genes in defined taxa. PolyPro consists of three modules: a GenBank Taxonomy Extractor (GTE), a Polynucleotide Probe Designer (PPD) and a Hybridization Parameters Calculator (HPC).

Download

The PolyPro is a freeware which runs on Windows platforms.

v1.8.4

Installion instructions

1. Download the ZIP file
2. Extract all files and folders from it (this is mandatory!)
3. Run PolyPro.exe

Article

Article: GeneFISH – an in situ technique for linking gene presence and cell identity in environmental microorganisms

Screen shots