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Hybridoma Center: Immunization Protocol

Washington University School of Medicine

Hybridoma Center
Department of Pathology
Center for Immunology

Immunization Protocol for Monoclonal Antibody Production

Antigens:

Adjuvant:

Schedule:

  Day   

                Manipulation                    

        Adjuvant       

          Site            
0 Primary Immunization CFA s.c.
14 Boost #1 IFA s.c.

24

 Boost #2 IFA s.c.
35 Boost #3 IFA i.v.
42 Bleed and test for antibody
45 Boost #4 IFA i.v.
66+ 3-4 week rest followed by i.v. or i.p. boost Saline/PBS i.v.
69 fusion 3d after i.v. boost or 4d after i.p. boost -- i.v.

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