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BioProtocol-DNA Molecular Weight Markers

DNA Molecular Weight Markers

Lambda DNA Hind III Digest
Lambda DNA EcoR I Digest
Lambda DNA Hind III/EcoR I Double Digest
Lambda BstE II Digest
pBR322 Msp I Digest
pBR322 BstN I Digest
φX174HaeIII Digest

*Lambda DNA Hind III Digest*
Fragment	Base Pairs
1	23,310
2	9,415
3	6,557
4	4,361
5	2,322
6	2,027
7	564
8	125

*Lambda DNA EcoR I Digest*
Fragment	Base Pairs
1	21,226
2	7,421
3	5,804
4	5,643
5	4,878
6	3,530

*Lambda DNA Hind III/EcoR I Double Digest*
Fragment	Base Pairs
1	21,226
2	5,148
3	4,973
4	4,268
5	3,530
6	2,027
7	1,904
8	1,584
9	1,375
10	947
11	831
12	564
13	125

*Lambda BstE II Digest*
Fragment	Base Pairs
1	8454
2	7242
3	6369
4	5686
5	4822
6	4324
7	3675
8	2323
9	1929
10	1371
11	1264
12	702
13	224
14	117

*pBR322 Msp* I Digest**
Fragment	Base Pairs
1	622
2	527
3	404
4	307
5	242
6	238
7	217
8	201
9	190
10	180
11,12	160
13,14	147
15	123
16	110
17	90
18	76
19	67
20,21	34
22,23	26
24	15

*pBR322 BstN I Digest*
Fragment	Base Pairs
1	1,857
2	1,058
3	929
4	383
5	121
6	13

φX174HaeIII Digest
Fragment	Base Pairs
1	1353
2	1078
3	872
4	603
5	310
6	281
7	271
8	234
9	194
10	118
11	72