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Appplication of Modifying Enzymes
New England Biolabs Technical Literature - Updated restriction_sm.gif (1260 bytes)
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Applications of Polymerases

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  Bst
DNA
L.Frag		 Taq Vent Vent
(exo-)		 Deep
Vent		 Deep
Vent
(exo-)		 9°Nm Ther-
minator		 T7
DNA 		E.coli
Poly I		 Klenow
Frag		 Klenow
exo-		 Reverse
Transc-
riptase		 T4
DNA.		 T7
RNA		 SP6
RNA
Labeling in vitro
3' fill-in  (+)  + (+) + (+) + (+)   (+) (+) (+) +   (+)    
3' exchange     +   +       + (+) +     +    
nick-translation   +               +            
replacement synthesis     (+)   (+)       (+) (+) (+)     +    
random priming + + + + + + +       + +        
RNA probes                             + +
Cloning
creating blunt ends
5' overhang fill-in     (+)   (+)   (+)   (+) (+) +     +    
3' overhang removal     +   +   (+)   (+) (+) +     +    
nested deletions                           +    
Sequencing (DNA)
DNA (+) (+)   (+)    (+)   +   (+) + (+) (+)      
RNA                         +      
Mutagenesis
oligonucleotide directed     (+)   (+)       +   (+)     +    
misincorporation   +                 +          
Mapping
transcript                         +      
cDNA
synthesis
1st strand                         +      
2nd strand (+)   (+)   (+)         (+) +          
cloning
Okayama-Berg                         +      
In vitro transcription                             + +

  +  = recommended enzyme for this application
(+) = usable, but not the enzyme of choice

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