This 180-page manual collects a wide variety of reliable protocols and technical tips and will enable you to quickly find the most optimal method for your specific needs.
Below is the table of contents. To select a chapter section, click on the corresponding page number. The chapters below are in Acrobat PDF format and must be viewed, searched, and printed with version 3.0 or above of the free Adobe Acrobat Reader
.
Chapter 1: Introduction
| Selection Chart | 3 | |
| Product Information and Major Subsequent Applications | 4 | |
| Overview of Nucleic Acid Purification and Isolation | 6 | |
| Methods described in this Manual | 7 |
Chapter 2: High PureTM Technology and Silica Adsorption
| Overview of Silica Adsorption | 10 | |
| High PureTM PCR Template Preparation Kit | 13 | |
| High PureTM PCR Product Purification Kit | 20 | |
| High PureTM Plasmid Isolation Kit | 27 | |
| High PureTM RNA Isolation Kit | 32 | |
| High PureTM Viral RNA Kit | 38 | |
| High PureTM Viral Nucleic Acid Kit | 42 | |
| General Troubleshooting Procedure for all High PureTM Kits | 46 | |
| PCR Clean Up Kit | 53 | |
| Agarose Gel DNA Extraction Kit | 58 |
Chapter 3: Solution-based Isolation
| Overview of Solution-based Isolation | 64 | |
| Split SecondTM DNA Preparation Kit | 66 | |
| DNA Isolation Kit for Mammalian Blood | 72 | |
| Plant DNA Isolation Kit | 79 | |
| TriPureTM Isolation Kit | 86 |
Chapter 4: Affinity Purification
Chapter 4a: Kits for Genetic Research
| Overview of Kits for Genetic Research | 94 | |
| mRNA Capture Kit | 96 | |
| mRNA Isolation Kit | 101 | |
| Triple HelixTM Plasmid Insert DNA Isolation Kit | 108 |
Chapter 4b: Kits for Isolation of mRNA for Biomedical Research
| Overview of mRNA Isolation Kits for Biomedical Research | 114 | |
| RNA/DNA Stabilization Reagent for blood/Bone Marrow | 118 | |
| mRNA Isolation Kit for Blood/Bone Marrow | 119 | |
| mRNA Isolation Kit for White Blood Cells | 126 |
Chapter 5: Gel Filtration
| Overview of Gel Filtration | 134 | |
| Quick SpinTM Columns for radiolabeled DNA/RNA purification | 136 | |
| Quick SpinTM Columns Linkers 6 | 140 |
Chapter 6: Premium Performance Products for PCR and RT-PCR
| Introduction | 146 | |
| TitanTM One Tube RT-PCR System | 147 | |
| Other Options for RT-PCR and Reverse Transcription | 148 | |
| ExpandTM PCR System | 149 | |
| ExpandTM High Fidelity | 150 | |
| ExpandTM Long Template | 151 | |
| ExpandTM 20 KbPLUS | 151 |
Chapter 7: Appendix
| Tips for Handling Nucleic Acids | 154 | |
| Conversion Tables and Formulas | 157 | |
| Buffers and Gels for Electrophoresis | 161 | |
| Fragment Sizes of DNA Molecular Weight Markers and Recommended Types of Agaroses | 163 | |
| Other Useful Information | 164 |
Chapter 8: Ordering Guide
| Ordering Guide | 168 |
Chapter 9: Index
| Index | 178 | |
| Disclaimers | ii |
| Editorial Management | Richard Bauer-Hofmann, Ph.D. | |
| Doris Eisel | ||
| Joe Keesey, Ph.D. | ||
| Balwant Patel, Ph.D. | ||
| Peter Rehberger | ||
| Doris Schmitt, Ph.D. | ||
| Ulrich Schuch, Ph.D. | ||
| Thomas Walter, Ph.D. |
| Art Direction and Typesetting: | typoPlus Foll + Schulz GmbH, Mannheim |