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Using FACS Scan Or FACS Calibur Machines (The Pamela Stanley Lab Wiki)
Guide on how to use FACS machines
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Sat Jan 31 2009, Hits: 809, Reviews: 0 Write review Cached
Standard Flow Cytometry (FACS) Protocol (Jackson Lab)
FACS Analysis Using Peripheral Blood Cells
http://www.jax.org/imr/facs.html
Added: Tue May 14 2002, Hits: 3137, Reviews: 0 Write review Cached
Yeast Prep for FACS Analysis (Gottschling Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/gottschling/Yeast%20Protocol...
Added: Fri Jul 26 2002, Hits: 436, Reviews: 0 Write review Cached
Cell Cycle Analysis by FACS (The Wolfson Institute for Biomedical Research)
This is the best guide on how to do cell cycle analysis on a FACS machine. It gives step by step instructions of how to adjust settings on the machine (this PDF file also appears here http://www.icmb.utexas.edu/core/Microscopy/pdf/CELL_CYCLE_FUNDAMENTALS.pdf)
http://www.ucl.ac.uk/wibr/services/docs/cellcyc.pd...
Added: Sat Feb 14 2009, Hits: 1188, Reviews: 0 Write review
Cytokines for FACS Analysis (The Science Advisory Board)
Protocol for intracytoplasmic staining of cytokines for FACS analysis
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 543, Reviews: 0 Write review Cached
FACS Machine Instructions (Koshland Web Site/Methods)
How to use FACS machine
http://www.ciwemb.edu/labs/koshland/Protocols/YEAS...
Added: Sat Feb 14 2009, Hits: 678, Reviews: 0 Write review Cached
FACS Troubleshootings (Protocol Online)
Archives of FACS troubleshootings
http://www.protocol-online.org/biology-forums/flow...
Added: Wed Feb 11 2009, Hits: 659, Reviews: 0 Write review Cached
A Rapid & Quantitative Method for Detecting Apoptosis by FACS in Transiently Transfected Cells (Nucl. Acids Res)
a method suitable for the flow cytometric determination of apoptosis in a large number of samples by propidium iodide (PI) in a population of cells transiently co-transfected with a plasmid encoding enhanced green fluorescent protein (eGFP) and a plasmid carrying a gene of interest. This assay combines the FACS-optimised GFP mutant (eGFP) as a marker to detect transiently transfected cells and the reduced fluorescence of the DNA-binding dye PI in the apoptotic subpopulation as a marker for apoptosis.
http://nar.oxfordjournals.org/cgi/content/full/25/...
Added: Fri Mar 20 2009, Hits: 342, Reviews: 0 Write review
FACS Aria Start Up (Cell Sorter Facility, Yale University)
http://info.med.yale.edu/immuno/cytometry/docs/FAC...
Added: Mon Feb 23 2009, Hits: 323, Reviews: 0 Write review Cached
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS) (The Pamela Stanley Lab Wiki)
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Sat Jan 31 2009, Hits: 1362, Reviews: 0 Write review Cached
Immunofluorescent Staining for FACS Analysis (Flow Cytometry Core Facility, PrincetonUniversity)
General immunofluorescence staining protocol using directly and indirectly conjugated antibodies
http://www.molbio1.princeton.edu/facility/flowcyt/...
Added: Sat Feb 21 2009, Hits: 661, Reviews: 0 Write review Cached
Preparing cells for PI/FACS (cell cycle) analysis (The Flemington Lab, Tulane Health Sciences Center)
http://www.flemingtonlab.com/Protocols/PreparingCe...
Added: Sat Jan 31 2009, Hits: 1109, Reviews: 0 Write review
FACS Analysis of ES Cells (NIH Stem Cell Information)
http://stemcells.nih.gov/research/nihresearch/scun...
Added: Thu Jan 29 2009, Hits: 346, Reviews: 0 Write review Cached
Cell Sorting By FACS (The Pamela Stanley Lab Wiki)
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Sat Jan 31 2009, Hits: 1741, Reviews: 0 Write review Cached
PI Labeling Method for FACS Analysis (Chen Lab, UCSF)
http://bts.ucsf.edu/chen/Protocol/PI%20Labeling%20...
Added: Sun Oct 25 2009, Hits: 222, Reviews: 0 Write review
Propidium Iodide Staining of Dead Cells For FACS Analysis (UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/pi.htm
Added: Sat Jan 31 2009, Hits: 1671, Reviews: 0 Write review Cached
FACS Procedures for Apoptosis Detection (University of Pennsylvania,SDM Flow Cytometry Facility )
Three methods are described: Hoechst vs. 7-AAD, TUNEL and Annexin V vs. 7-AAD
http://biochem.dental.upenn.edu/~sdmfacs/apopproc....
Added: Sat Jan 31 2009, Hits: 1392, Reviews: 0 Write review Cached
FACS Cell Cycle Staining using DAPI (Flow Cytometry Core Facility, University of Chicago)
http://iacf.uchicago.edu/Protocols/DAPICellCyclePr...
Added: Sat Feb 21 2009, Hits: 816, Reviews: 0 Write review Cached
CD3/ CD4 /CD8 T Cell Subset Counts by FACS (The Science Advisory Board)
Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 1414, Reviews: 0 Write review Cached
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells (Journal of Visualized Experiments)
A video and text protocol for differentiating mouse ES cells including the following procedures: Assembling handing drops differentiation culture (EB formation), Collect undifferentiated ES cells, Assemble hanging drop culture, Collecting differentiated ES cells form hanging drops, Starting EB culture, EB dissociation and infection, Preparation of ES cell-derived progenitors for FACS and Transplantation.
http://www.jove.com/index/Details.stp?ID=162
Added: Wed Jan 28 2009, Hits: 323, Reviews: 0 Write review Cached
Intracellular Staining Protocol (Flow Cytometry Core Facility, University of Chicago)
Staining of intracellular marker for FACS analysis
http://iacf.uchicago.edu/Protocols/IntracellularSt...
Added: Sat Feb 21 2009, Hits: 1040, Reviews: 0 Write review Cached
FACSCalibur Set-up Using CellQuest Pro Software (Flow Cytometry Core Facility, Medical University of South Carolina)
Step by step illustrated guide on using FACS machine
http://hcc.musc.edu/research/sharedresources/flowc...
Added: Sat Feb 14 2009, Hits: 747, Reviews: 0 Write review