Protocol Matches: 282

• Site-directed Mutagenesis using PCR (Lazo Lab)
  PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.
  http://wheat.pw.usda.gov/~lazo/methods/lazo/pcr-mu...
  Added: Tue May 14 2002, Hits: 1700, Reviews: 0  Write review   Cached
• Single Tube Confirmation PCR Protocol (Saccharomyces Genome Deletion Project)
  A colony PCR strategy is used to identify successful deletions. This method is fast, reliable, and amenable to a 96-well format
  http://sequence-www.stanford.edu/group/yeast_delet...
  Added: Tue May 14 2002, Hits: 857, Reviews: 0  Write review   Cached
• TA Cloning and Vector Preparation (Janos Luka Ph.D., Department of Pathology, Eastern Virginia Medical School)
  It is relatively easy and cheap to make a vector containing a T overhang. Because Taq polymerase ads an A overhang to PCR products in standard PCR assays, almost any PCR product can be cloned into this vector. The purified T-vector can be stored aliquoted at -70 C for at least one year. This protocol describes how to make T vectors and how to clone PCR products.
  http://herpesvirus.tripod.com/research/T-vectorclo...
  Added: Fri Apr 16 2004, Hits: 3802, Reviews: 0  Write review   Cached
• PCR Product Cleanup by Ethanol Precipitation (Ortí Laboratory at the School of Biological Sciences, University of Nebraska-Lincoln)
  Ethanol precipitation method for purifying PCR products
  http://golab.unl.edu/protocols/PCR-EtOH.html
  Added: Fri Feb 13 2009, Hits: 7676, Reviews: 0  Write review  
• PCR Product Purification or Clean Up (Wei-Shou Hu Laboratory,University of Minnesota)
  PCR cleanup method without using a kit.
  http://hugroup.cems.umn.edu/Protocols/Protocols%20...
  Added: Fri Feb 13 2009, Hits: 5852, Reviews: 0  Write review  
• Important Parameters of Quantitative PCR (qPCR) Analysis (Life Technologies)
  Very useful information about qPCR such as amplification efficiency, Setting the Baseline, Determination of Copy Number,Tm Calculations, PCR Optimization, etc.
  http://www.invitrogen.com/site/us/en/home/Referenc...
  Added: Thu May 02 2013, Hits: 3240, Reviews: 0  Write review   Cached
• PEG Purification of PCR Products (GSCWU)
  This protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing
  http://genome.wustl.edu/tools/protocols/index.php?...
  Added: Tue May 14 2002, Hits: 1434, Reviews: 0  Write review   Cached
• PCR (Bowtell Lab)
  http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
  Added: Tue May 14 2002, Hits: 788, Reviews: 0  Write review   Cached
• In Situ PCR On Plant Tissues (Bo Johansen)
  Provides detailed protocol on tissue preparation, in situ PCR, detection and required reagents.
  http://www.bot.ku.dk/staff/boj/insitupcr.htm
  Added: Tue May 14 2002, Hits: 180, Reviews: 0  Write review   Cached
• Colony Screening by PCR (Matt Lewis, Department of Pathology, University of Liverpool)
  This is the fastest way to screen bacterial colonies
  http://www.methodbook.net/pcr/pcrscreen.html
  Added: Fri Sep 06 2002, Hits: 3247, Reviews: 0  Write review   Cached
• PCR Primer Design (Eppendorf)
  This application note is provided to give rules that should be taken into account when designing primers for PCR.
  http://www.brinkmann.com/PCR_appl_primer.asp
  Added: Tue May 14 2002, Hits: 820, Reviews: 0  Write review   Cached
• Detect Alu Repeat by PCR (Biotech)
  PCR is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene.
  http://www.accessexcellence.com/AE/AEPC/DNA/detect...
  Added: Tue May 14 2002, Hits: 1668, Reviews: 0  Write review   Cached
• Multiplex PCR: critical parameters and step-by-step protocol. (Biotechniques)
  A protocol for developing a multiplex PCR assay and suggest ways to overcome commonly encountered problems.
  http://www.biotechniques.com/multimedia/archive/00...
  Added: Sat Feb 16 2013, Hits: 5086, Reviews: 0  Write review  
• 5' RACE (Anchor-ligated PCR) (Langdale Lab, Department of Plant Sciences, University of Oxford)
  This protocol uses either oligo d(T) or a gene specific primer (priming from 3’ to 5’) to make cDNA. After RNA hydrolysis, a single stranded adaptor is ligated to the 5’ end of the single stranded cDNA. Amplification is subsequently performed using primers to the adaptor and a gene specific primer (which can be the same as that used in the first step).
  http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
  Added: Wed Feb 11 2009, Hits: 3015, Reviews: 0  Write review  
• PCR clean up using 3M sodium acetate and chilled absolute ethanol (KAIMRC, NGHA )
  Clean up PCR in 1.5 hrs. with a very easy steps.
  Added: Sat Jan 02 2010, Reviews: 0  Write review  
• Degenerate PCR (Michael Koelle, at another server)
  Detailed guide in degenerate PCR, including primer design, reaction conditioning and product analysis.
  http://info.med.yale.edu/mbb/koelle/protocols/prot...
  Added: Tue May 14 2002, Hits: 4763, Reviews: 0  Write review   Cached
• Preparation of Mouse Tail DNA for Dot Blots or PCR  (Eric Mercer)
  These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subsequently modified for PCR preps.
  http://www.muridae.com/wmc/docs/tailDNA_prep.html
  Added: Tue May 14 2002, Hits: 616, Reviews: 0  Write review   Cached
• PCR Manual (BMB)
  This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.
  http://206.53.227.20/prod_inf/manuals/pcr_man/star...
  Added: Tue May 14 2002, Hits: 2822, Reviews: 0  Write review   Cached
• Degenerate PCR Primer Design (Molecular Biology Techniques Manual)
  For amplification of cognate sequences from different organisms, or for "evolutionary PCR", one may increase the chances of getting product by designing "degenerate" primers: these would in fact be a set of primers which have a number of options at several positions in the sequence so as to allow annealing to and amplification of a variety of related sequences.
  http://web.uct.ac.za/microbiology/pcroptim.htm#Deg...
  Added: Tue May 14 2002, Hits: 5064, Reviews: 0  Write review   Cached
• Adjuvants in PCR Reactions (Octavian Henegariu, Yale University)
  Use of DMSO, glycerol and BSA in PCR reaction.
  http://info.med.yale.edu/genetics/ward/tavi/p16.ht...
  Added: Thu Apr 22 2004, Hits: 4401, Reviews: 0  Write review   Cached
• Purification of PCR products (Srel DNA Lab, University of Georgia)
  PEG Cleaning of PCR products prior to sequencing
  http://www.uga.edu/srel/DNA_Lab/PEG_Precip'00.rtf
  Added: Mon Sep 23 2002, Hits: 2636, Reviews: 0  Write review   Cached
• Real-time PCR in virology (Dr. Michael W. Pfaffl, Gene Quantification )
  This paper describes the background, advantages and limitations of real-time PCR
  http://www.wzw.tum.de/gene-quantification/mackay-2...
  Added: Sun Sep 22 2002, Hits: 6157, Reviews: 0  Write review  
• Real-Time PCR Handbook (RRC Core Genomics Facility, University of Illinois at Chicago)
  Covers real-time PCR theories, methods, primer design, etc
  http://www.uic.edu/depts/rrc/cgf/realtime/start.ht...
  Added: Thu Feb 05 2009, Hits: 15454, Reviews: 0  Write review   Cached
• Digestion of PCR Products (Fermentas)
  Some basic facts you need to know to cut your PCR products.
  http://www.fermentas.com/techinfo/RE/restrdigpcr.h...
  Added: Tue May 14 2002, Hits: 366, Reviews: 0  Write review   Cached
• PCR of DNA from Ear Clip Tissue (Transgenic and ES Cell Services at OSU)
  http://www.med.ohio-state.edu/tafhome/webpages/pro...
  Added: Tue May 14 2002, Hits: 108, Reviews: 0  Write review   Cached

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