Protocol Matches: 282
- RT-PCR Primer Design (Roche Diagnostics Corporation)
How to design RT-PCR primers allowing differentiation between the amplified product of cDNA and an amplified product derived from contaminating genomic DNA.
Added: Sat Sep 21 2002, Hits: 893, Reviews: 0
- Quick and Easy Isolation of Genomic DNA from Yeast (Protocol Online)
This protocol describes a quick and easy method for genomic DNA preparation from yeast. The protocol can be used for PCR or southern blot analysis
Added: Mon Feb 02 2009, Reviews: 0
Nested RT-PCR From Paraffin Section
This protocol is for the non-isotopic detection of hepatitis C RNA and albumin mRNA (as an internal control) from 4 micron sections of formalin-fixed, paraffin-embedded liver biopsies by RT-PCR. The procedure for RNA extraction from formalin fixed paraffin embedded section and PCR amplification is described.
Added: Tue May 14 2002, Hits: 299, Reviews: 0
- In Situ RT-PCR (Sinha Lab, University of California, Davis)
Detailed protocol on in situ PCR on tissue samples including procedures for tissue preparation.
Added: Wed Sep 04 2002, Hits: 4036, Reviews: 0
Non-organic Isolation of Tail DNA
Using tail tissue directly for PCR after proteinase K digestion
Added: Tue May 14 2002, Hits: 831, Reviews: 1 Read reviews
- Isabelís Magical Cloning Protocol (Lahn's Lab, University of Chicago)
Describes the whole molecular cloning procedures from PCR amplification, vector and insert digestion, vector dephosphorylation, ligation, transformation, plasmid preparation and screening to plasmid sequencing.
Added: Fri Mar 06 2009, Hits: 6759, Reviews: 0
Processing of Microdissected Tissue for DNA Analysis
(Molecular Profiling Initiative, NCI)
Process micordissected tissue for DNA analysis such as PCR
Added: Tue May 14 2002, Hits: 223, Reviews: 0
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