| Product Description | Dry or fresh plant tissue is disrupted and then lysed in a special buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by isopropanol precipitation of DNA. Binding conditions are then adjusted. The sample is then applied to a spin column and centrifuged. DNA binds to the silica membrane while contaminants such as proteins and polysaccharides are efficiently removed by two wash steps. Purified DNA is eluted in a small volume of low ionic strength buffer or water.
Feature and benefits
Rapid, DNA isolation under 60 min
Stable, high-quality silica membrane and ideal buffer system ensure the reproducible results
High purity, Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9
Isolated DNA ranges from 30Kb to 50Kb and can be directly used for most downstream applications, including PCR, Southern-blot, Restriction digestion reactions, etc. |
