Product Name | NEXTflex™ Small RNA-Seq Kit v2 |
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Product Description | The patent pending NEXTflex™ Small RNA Sequencing Kit v2 provides an user-friendly, flexible, cost-effective solution for generating small RNA libraries with reduced bias for sequencing on Illumina platforms. Bioo Scientific’s proprietary approach to overcoming ligation bias in sRNA-seq libraries involves using a pool of adapters having randomized sequences at the ligation site, where each adapter sequence is present in vast molar excess over any given small RNA in the sample. Experiments show that most of the bias in adapter ligation is due to the sequence of 2-4 adapter nucleotides adjacent to the target junction. No single adapter sequence is able to efficiently ligate to all small RNAs, indicating that the target sequence, as well as the adapter sequence, is a source of bias. The new NEXTflex Small RNA Sequencing Kit v2 uses a randomized adapter strategy to allow small RNAs of any sequence to “find” their optimal corresponding adapters, resulting in sRNA-seq libraries that show a dramatic reduction in bias. |
Product Manual | Read Product Manual |
Price | $2549 |
Specification | 48 rxns |
Product Review | 0 |
Vendor Information | Bioo Scientific www.biooscientific.com 7050 Burleson Rd Austin TX 78744 USA Phone: 1-888-208-2246 E-Mail: info@biooscientific.com |