Product Description | The Kit applies the unique binding buffer/ Proteinase K to rapidly lyse cells and inactivate cellualar nuclease, then DNA selectively absorbed on silica membrane in high salt solution, after that take a serial of elution- centrifugation step to remove cellular metabolite and proteins etc. Finally use low salt elution to elute purified genome DNA from silica membrane.
Features
The silica membrane in the spin-column comes from the world-famous company.
Poisonous phenol etc not used.
Procedure is simple and fast, single sample can be completed in 30 min.
Multi-elution can ensure high-purified DNA, the typical ratio of OD260/OD280 is 1.7¡«1.9, and the average length up to 30Kb-50kb, which can be applied for PCR£¬Southern-blot and digestions directly. |