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Restriction enzyme Related Discussions
  1. detection of restriction fragments - restriction fragments (reply: 1)
  2. Tool to investigate cleavage sites within a protein - (reply: 2)
  3. BamHI/XhoI ligation - (reply: 3)
  4. Double Digestion Restriction Enzyme - Troubles with double digestion (reply: 3)
  5. cloning vector and restriction mapping - (reply: 2)
  6. restriction enzyme digestion - (reply: 5)
  7. Double restriction digestion - (reply: 13)
  8. Restriction Mapping - Need help in clearing concept (reply: 1)
  9. Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
  10. Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
  11. RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
  12. restriction enzymes that cut at methylated cytosines - (reply: 2)
  13. DNA enzyme digestion problems - Smeared and stuck in wells, no or faint bands (reply: 1)
  14. dam methylation? - digestion problem (reply: 3)
  15. genomic DNA digestion - EcoRI+MseI - (reply: 2)
  16. double digestion time - (reply: 1)
  17. deisgning primers with restriction site - (reply: 7)
  18. Sequential digestion protocol - (reply: 3)
  19. precipitation during TEV cleavage - (reply: 1)
  20. Pulse Field Gel Electrophoresis (PFGE) - Secondary Restriction Enzyme Problem (reply: 2)
  21. In frame clonning adding a restriction site - (reply: 1)
  22. genomic library - use of restriction enzymes (reply: 10)
  23. Restriction enzymes not cutting - (reply: 4)
  24. Restriction Enzyme digestion - (reply: 4)
  25. Mysterious Band in NdeI/BamHI Double Digest - (reply: 7)
  26. failed restriction digests - (reply: 11)
  27. digestion at 4 degrees - (reply: 6)
  28. killer digest issues - (reply: 8)
  29. Xho1/Bglll digest of pSUPER.retro.puro with stuffer problems - (reply: 1)
  30. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
  31. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
  32. chromatin DNA and restriction endonucleases - (reply: 1)
  33. Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
  34. Why the addition of restriction sites to primers? - (reply: 3)
  35. No band is visible with BamHI and XbaI Digestion - (reply: 3)
  36. restriction enzyme sites - disruption (reply: 4)
  37. Digest with BglII and SalI - (reply: 1)
  38. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  39. plasmid digestion gel - (reply: 4)
  40. protein precipitation after enterokinase cleavage - (reply: 1)
  41. restriction digestion of plasmid - (reply: 8)
  42. Two restriction sites are close each other - Can I use them?? (reply: 3)
  43. Genome Restriction Digestion - (reply: 2)
  44. Digest Buffer Precipitation - Na2HPO4, L-Cysteine, EDTA (reply: 4)
  45. Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
  46. Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
  47. proteolytic cleavage - proteolytic cleavage (reply: 1)
  48. M13 Hind and Sal digest - (reply: 2)
  49. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
  50. Smart Sofatware for Restriction Analysis - multiple simultaneous restriction analysis... (reply: 10)
  51. lux gene cloning using Sal I as restriction enzyme - (reply: 1)
  52. Sequencing incorrect after digestion and ligation - (reply: 5)
  53. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
  54. Tool for complementary restriction enzymes? - REs producing overhangs to religate (reply: 5)
  55. digestion of pcr - (reply: 2)
  56. double digestion and ligation - (reply: 3)
  57. Restriction Enzyme Troubleshooting Help? - (reply: 2)
  58. Help restriction analysis of plasmid - (reply: 11)
  59. promoter pcr using primers with restriction sites giving trouble - (reply: 7)
  60. Aniline cleavage of RNA - (reply: 1)
  61. Thrombin digestion for GST - ppt problem (reply: 3)
  62. Multimerization after restriction digestion - (reply: 9)
  63. restriction enzyme digestion problem - (reply: 8)
  64. Help, sequestial digest - (reply: 1)
  65. help. Is this double digest right? - (reply: 5)
  66. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
  67. Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
  68. Double digest and 3' end polishing with klenow - (reply: 1)
  69. Mystery band after Phenol:Chloro:IAA Extraction after restriction digest - (reply: 3)
  70. methylated restriction sites - (reply: 1)
  71. caspase-3 cleavage assay - how to test the clevage of a protein by caspase in vitro (reply: 2)
  72. how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
  73. Why does insert has two bands after RE digestion? - (reply: 3)
  74. No band after Double digestion - (reply: 4)
  75. PGEM-T easy gel - partial digestion? - (reply: 2)
  76. Primer design: how do I add a restriction site to my primer? - (reply: 2)
  77. Miniprep DNA digestion smear - (reply: 15)
  78. restriction enzyme - can i cut the noncoding region? - (reply: 2)
  79. how to insert a thrombin cleavage site - ....into my vector (reply: 3)
  80. only one band after double enzyme digestion - (reply: 4)
  81. Restriction Enzyme-EcoR V come frozen !? - (reply: 6)
  82. How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
  83. all the DNA gone after digestion - (reply: 8)
  84. EcoR1-Pst1 digest for vector and insert - (reply: 3)
  85. Vector digestion - (reply: 6)
  86. how to do double digestion with EcoRI and BamHI - (reply: 9)
  87. How to get rid the restriction sites in a plasmid ? - (reply: 5)
  88. NEB restriction enzyme BssS1 - digest (reply: 1)
  89. thrombin cleavage buffer - (reply: 1)
  90. General question about restriction sequences in PCR primers - (reply: 3)
  91. no band after digestion - (reply: 5)
  92. BamH1-Pst1 digest - (reply: 5)
  93. problems with restriction digestion - (reply: 7)
  94. how to add bases to the primer restriction sites - (reply: 2)
  95. southern blot - southern blot- restriction of genomic DNA (reply: 2)
  96. problem with restriction digest - I have an insert, but no vector.... (reply: 5)
  97. Restriciton digest protocol for adjacent sites? - (reply: 7)
  98. using restriction enzymes for studying DNA methylation - hemimethylation (reply: 3)
  99. Ecor1/Pst1 digest - (reply: 4)
  100. For double sequential digest: which enzyme first? - (reply: 7)
  101. Problems with double digestion - (reply: 1)
  102. restriction enzyme site with primers - (reply: 4)
  103. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
  104. Extremely Low Yield PCR with restriction site addition - Assistance requested for beginning molecular biologist on PCR techniqu (reply: 3)
  105. can hyaluronidase be used to detach/digest cells? - (reply: 1)
  106. Dnase digestion after in vitro transcription - (reply: 4)
  107. How important is Ca2+ to thrombin cleavage? - (reply: 2)
  108. DNA smears after digestion - DNA smears after digestion (reply: 6)
  109. RE digestion Help needed everyone - (reply: 6)
  110. P/C/I extraction of plasmid after digestion - (reply: 1)
  111. double digestion of transformed bacteria colony - (reply: 7)
  112. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  113. Amt of restriction enzyme - (reply: 4)
  114. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
  115. Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
  116. how to check if restriction digestion has happened - (reply: 7)
  117. Restriction enzyme sites mutate after ligation? - (reply: 2)
  118. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  119. Digestion dilemma - (reply: 5)
  120. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
  121. Cloning PCR product blunt + restriction site - (reply: 2)
  122. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
  123. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
  124. smearing in the restriction digested products - (reply: 2)
  125. DNA degradation while digestion. - (reply: 3)
  126. HELP in Restriction Enzyme Digestion - troubleshooting re digestion (reply: 3)
  127. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
  128. Proteases and thier site cleavage - Enzyme digestion (reply: 2)
  129. Problem with restriction enzyme NdeI - How many bases do I need? (reply: 6)
  130. Digestion question - (reply: 1)
  131. Integrity of overhang after digestion - (reply: 3)
  132. PstI/SapI digestion problem - restriction enzyme (reply: 3)
  133. How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
  134. function of restriction enzymme - (reply: 2)
  135. Double restriction enzyme digest - (reply: 3)
  136. NheI, EcoRI vector double digest - Troubling getting second enzyme to cut (reply: 6)
  137. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
  138. SacII digestion - (reply: 1)
  139. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  140. Restriction Enzyme Binding - (reply: 3)
  141. NotI / EcoRI digest - (reply: 5)
  142. Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
  143. DNA loss in digestion - I lose lot of DNA in digestion (reply: 10)
  144. pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
  145. Problems with restriction enzyme digest. - (reply: 2)
  146. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
  147. Restriction sites too close? - (reply: 2)
  148. Restriction digested plasmid - (reply: 2)
  149. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
  150. Closing up restriction sites - KpnI/XhoI site needs to get closed (reply: 2)
  151. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
  152. DNase Digestion and Reverse Transcription - (reply: 10)
  153. Restriction Fragment (urgent) - (reply: 4)
  154. Double digestion - (reply: 13)
  155. double digestion - (reply: 6)
  156. unable to digest my recombinant plasmid! - (reply: 9)
  157. Double Digestion with Single Restriction Enzyme - (reply: 2)
  158. plasmid DNA Digestion - (reply: 5)
  159. NsiI digestion problems - NsiI is not working properly? (reply: 1)
  160. Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
  161. problem with restriction digestion - (reply: 3)
  162. restriction digestion - (reply: 5)
  163. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
  164. Introduce restriction sites with PCR primers - (reply: 2)
  165. Restriction enzyme digestion problem - (reply: 4)
  166. Concerning digestion enzymes - How do digestion enzymes work really? (reply: 1)
  167. Why should it be digested with a restriction enzyme before treated with bisulfi - (reply: 5)
  168. partial digestion of Tth111I - difficult??? (reply: 2)
  169. Where exactly does a restriction enzyme cut in its recognition site? - (reply: 1)
  170. Restriction of pichia vector pPICZ alpha with Xho1 and Xba1 - (reply: 1)
  171. Protein cleavage site? - (reply: 1)
  172. How can I delete a fragment that is not surrounded by restriction sites? - (reply: 3)
  173. partial digestion of Tth111I - (reply: 1)
  174. Question about Restriction enzyme - (reply: 3)
  175. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
  176. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
  177. Problem with Digestion or Ligation. How to tell? - (reply: 7)
  178. urgent HincII digestion problem - (reply: 5)
  179. Cloning help - how to break down a restriction site! (reply: 6)
  180. Digestion in sub-optimal buffer - (reply: 5)
  181. Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
  182. Double RE digest: using two different companies - (reply: 3)
  183. Double digest question for ligation - (reply: 4)
  184. Restriction mapping - (reply: 2)
  185. Restriction fragment length polymorphism - (reply: 1)
  186. Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
  187. Do restriction enzymes work on dUTP? - (reply: 1)
  188. Destruction of restriction site - (reply: 1)
  189. Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
  190. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
  191. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
  192. Protecting against Bgl II digestion - (reply: 2)
  193. Restriction digestion - (reply: 2)
  194. restriction enzymes primers - (reply: 5)
  195. Kpn1 restriction enzyme - (reply: 12)
  196. Sequential digestion - (reply: 3)
  197. What is wrong with my EcoRI digestion? - Can it attribute to the star activity? (reply: 6)
  198. Can restriction digestion be replaced by CHIP? - (reply: 5)
  199. Restriction digest of pQE plasmid - Double digestion problem (reply: 7)
  200. Digestion, Ligation or transformation problem? - (reply: 8)
  201. double digestion of plasmid - problem with incomplete digestion (reply: 9)
  202. ChIP- any experiences in using restriction enzymes instead of sonication? - (reply: 4)
  203. Nla III digestion - (reply: 2)
  204. Protein purification after cleavage (thioredoxin) - (reply: 3)
  205. is apoptosis always parp cleavage dependent? - cancer-parp-zvad-flow cyto (reply: 2)
  206. Vector with many restriction site - (reply: 6)
  207. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  208. Inhibition of BamHI - (reply: 4)
  209. cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
  210. Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
  211. enygmatic restriction by BamH1 - (reply: 8)
  212. Phusion kit altering my restriction sites only - (reply: 5)
  213. Restriction with two incompatible enzymes - (reply: 4)
  214. Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
  215. Restriction site - (reply: 3)
  216. double digestion with BamHI and HindIII - (reply: 13)
  217. Anyone used restriction enzyme BsaI? - (reply: 3)
  218. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
  219. RNAse and DNA digestion in Southern Blot - (reply: 3)
  220. I need help with the steps in sequential digests please - (reply: 3)
  221. BAC DNA restriction analysis - I need help :( (reply: 4)
  222. restriction map - (reply: 7)
  223. Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
  224. non-specific cleavage products in an endonuclease assay - (reply: 3)
  225. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
  226. Can any one help me with restriction digestion map for DNA? - (reply: 3)
  227. Is a Triple Restriction Digest Possible? - (reply: 6)
  228. Clonning with one restriction enzyme - Some help please (reply: 2)
  229. Restriction digestion of PCR product - (reply: 3)
  230. Restriction enzyme specificity for thymine and DNA - are thymine/uracil and DNA/RNA interchangeable? (reply: 1)
  231. AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
  232. Is restriction enzyme Srf1 a good cutter? - (reply: 4)
  233. Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
  234. Problem on restriction digest - Inquire (reply: 11)
  235. ppt DNA directly from a digestion/ pcr - (reply: 2)
  236. Restriction digest - volume of restriction digest (reply: 2)
  237. RE digest and klenow - sequential blunting (reply: 6)
  238. Help with AVRII digestion - (reply: 1)
  239. Restriction enzyme -Eco47III - (reply: 1)
  240. his-tag thrombin cleavage in-column? - as a purification strategy (reply: 1)
  241. which restriction enzyme should I use to digest the vector? - (reply: 3)
  242. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  243. restriction site in primers urgent - (reply: 1)
  244. Methylated DNA Interfering with Restriction Digestion - Restriction digestion problems (reply: 3)
  245. Is my restriction digest not cutting properly? - (reply: 8)
  246. Restriction site clonning failed - (reply: 9)
  247. Primer design with restriction sites - (reply: 3)
  248. can I take some of ligation mix and digest? - (reply: 4)
  249. Restriction Enzyme and Sequencing Problems - (reply: 1)
  250. restriction enzymes - (reply: 4)
  251. PCR band for water but no contamination, Digestion not working - (reply: 10)
  252. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
  253. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
  254. double restriction enzyme digestion - not work? (reply: 6)
  255. gfp digested with Ecor1 + Hind III, and Bgl II - (reply: 4)
  256. Restriction digestion - (reply: 7)
  257. My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
  258. 3 bands after digestion - (reply: 13)
  259. thrombin cleavage and triton? - protein purification (reply: 2)
  260. Southern Blot: which restriction enzyme? - (reply: 1)
  261. Hepg2 cells resistant to trypsin digestion - (reply: 7)
  262. Restriction digestion by SpeI and SphI - (reply: 2)
  263. PacI digestion - (reply: 3)
  264. please tell me more about primers with restriction site! - (reply: 4)
  265. Restriction sites frequencies in mouse genome - (reply: 10)
  266. restriction of Open Reading Frame (ORF) - (reply: 1)
  267. Few colonies with cloning of PCR product after digestion - (reply: 7)
  268. TM for primers with restriction secuence tail. - Suggestion (reply: 3)
  269. digesting out restriction site - (reply: 6)
  270. Can DNA ethanol precipitation remove restriction enzyme? - (reply: 7)
  271. Religation with single digest vector - (reply: 7)
  272. Identify insert by restriction digestion - (reply: 6)
  273. Afl II linearization & ligation - (reply: 3)
  274. Problems with SalI digestion - (reply: 5)
  275. How to add restriction enzyme sites to the primer - (reply: 6)
  276. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
  277. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
  278. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
  279. restriction direct to ligation? - skip clean up? (reply: 12)
  280. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  281. Cleavage of target protein from glutathione sepharose - (reply: 3)
  282. Tryptic digest help - Trouble diffusing stain (reply: 3)
  283. Factor Xa Cleavage - MBP-Factor Xa Cleavage (reply: 2)
  284. Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
  285. Problem with DNase digestion of RNA - (reply: 6)
  286. RE digest and subsequent ligation - A base is now missing from restriction site - how, why? (reply: 7)
  287. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
  288. trouble with restriction digestion - (reply: 3)
  289. adding restriction sites to degenerate primers - (reply: 9)
  290. BAC digestion - (reply: 8)
  291. Restriction digest as a template - (reply: 2)
  292. restriction to ligation - (reply: 5)
  293. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  294. Problem with BamHI/SalI double digestion - (reply: 8)
  295. disappearing vector after digestion - (reply: 11)
  296. restriction digestion of PCR product? - (reply: 10)
  297. Removing vector restriction sites - and replacing with new ones (reply: 8)
  298. Stuborn band after digestion of plasmid DNA - (reply: 4)
  299. double digestion problem - additional band after double digestion (reply: 2)
  300. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  301. NdeI double digestion - (reply: 3)
  302. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
  303. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
  304. Restriction buffer - (reply: 3)
  305. problems with NdeI restriction enzyme? - (reply: 2)
  306. is there a restriction about amount of vector in a ligation? - (reply: 14)
  307. Full and Partial Digestion - (reply: 3)
  308. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
  309. Basic Question Restriction Digest - (reply: 11)
  310. ApaI / Not I double digestion problem - (reply: 6)
  311. XbaI/BamHI double digest- dam methylation detection problem - (reply: 3)
  312. Digestion Problem with BsmBI - (reply: 3)
  313. Vortexing restriction digests? - (reply: 5)
  314. If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
  315. Cloning a PCR product after digestion - (reply: 7)
  316. Question about proteinase cleavage sites - (reply: 1)
  317. dimers of dna (not primers) following digest or pcr? - (reply: 11)
  318. KpnI and BamHI double digest help - (reply: 6)
  319. cloning restriction fragment for sequencing - (reply: 1)
  320. reverse primer design with restriction site - (reply: 6)
  321. Pronase digestion - (reply: 1)
  322. stability of protein sample after in-gel digestion - (reply: 2)
  323. Would ExoI work? - Alternative nuclease for DNA digestion (reply: 1)
  324. QIAquick PCR for preparing for restriction digestion - very low concentrations (reply: 4)
  325. Unique restriction sites in two sequences - (reply: 3)
  326. How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
  327. peptidase cleavage sites - (reply: 3)
  328. problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
  329. website for identifying restriction sites in a gene - (reply: 6)
  330. southern and restriction enzyme - what enzyme to choose (reply: 6)
  331. sequencing a restriction fragment - (reply: 4)
  332. Restriction Analysis - (reply: 1)
  333. keep getting incomplete digest for plasmid - (reply: 3)
  334. Insertions/deletions at cloning restriction site - (reply: 1)
  335. double digest pcDNA(+) by XhoI&XbaI, uncompletely? - (reply: 3)
  336. CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
  337. How to fill in 5` overhangs after Restriction digestion - (reply: 2)
  338. Effect of bile concentration on human digestion - Human digestion (reply: 5)
  339. Role of BSA in restriction enzyme digestion - help (reply: 2)
  340. restriction digestion problems - digestion problems with SalI (reply: 2)
  341. Reagents used...in restriction digestion and electrophoresis - (reply: 2)
  342. protein digestion - (reply: 1)
  343. restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
  344. trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
  345. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  346. do you sonicate or digest with lysozyme? - which one do you prefer and why.... (reply: 21)
  347. Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
  348. How does normal mRNA escape from cleavage by dicer? - (reply: 5)
  349. Is A-tailing of restriction fragment possible? - (reply: 2)
  350. Lipase enzyme digestion of milk experiment - effects of stale milk - Help needed understanding how stale milk could affect my experiment... (reply: 2)
  351. Help on digestion and ligation...colonies not wanted why? - (reply: 4)
  352. problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
  353. How to extract a 20kb vector after double digestion - (reply: 4)
  354. double digestion and dephosphorylation - (reply: 5)
  355. His tag cleavage by Thrombin - (reply: 2)
  356. Restriction digest - (reply: 6)
  357. Restriction enzyme that cut CGCGCG? - (reply: 3)
  358. Interpreting RE digest results after blunt cloning - (reply: 1)
  359. No band on double digestion of construct - (reply: 1)
  360. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
  361. BclI digestion - (reply: 4)
  362. Digestion Problem - Cloning (reply: 5)
  363. basic RE digests acting weird - (reply: 3)
  364. Nhe1 Restriction digestion - (reply: 3)
  365. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  366. tricky digestion for cloning gurus - (reply: 13)
  367. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
  368. EcoRI problem - (reply: 4)
  369. partial digestion problem - (reply: 8)
  370. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  371. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  372. Cloning - RE digestion clean up (reply: 2)
  373. do primers and dNTPs alter digestion efficiency? - (reply: 4)
  374. Enzyme Restriction - (reply: 4)
  375. Incomplete genomic digestion for Southern - (reply: 1)
  376. Unexplained His-tag cleavage - (reply: 2)
  377. pQE30 sequence needed - or restriction map (reply: 3)
  378. Plasmid appears as two bands after double digestion - (reply: 2)
  379. attach adaptors or PCR the restriction enzyme sites? - which one would you suggest and why (reply: 2)
  380. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  381. Partial digest to destroy an enzyme site - (reply: 10)
  382. CIP with restriction digestion - (reply: 5)
  383. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
  384. Use EcoRI/KpnI, good combination? - (reply: 4)
  385. HindIII digestion - (reply: 1)
  386. how many bases are needed upstream of restriction site? - (reply: 5)
  387. Restriction with pstI - (reply: 1)
  388. restriction enzyme specificity - (reply: 7)
  389. Bisulfite conversion - necessary to digest DNA? - (reply: 1)
  390. BamHI/XbaI cloning, bad combination? - (reply: 3)
  391. GST Tag Cleavage - (reply: 2)
  392. how do you inactivate the enzymes after digestion? - (reply: 17)
  393. PCR primer: 2 restriction sites? - (reply: 4)
  394. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
  395. HELP! selective scission - thrombin digestion (reply: 1)
  396. gel purification after restriction digestion - (reply: 3)
  397. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
  398. How 3M KAc to influence restriction enzyme activity? - (reply: 4)
  399. Restriction map of pUC18 - (reply: 2)
  400. why we use BVS in DNA digestion? - (reply: 3)
  401. Retroviral Vector won't digest ideas! - (reply: 1)
  402. restriction digestion problem... - (reply: 12)
  403. unpredictable plasmid - can digest but not sequence. . . (reply: 4)
  404. Problem with BsaB1 digestion - (reply: 1)
  405. how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
  406. Southern - Southern Blot Digestion (reply: 4)
  407. how do you check your restriction enzyme is working? - (reply: 12)
  408. Role of restriction and TAE Buffer - (reply: 1)
  409. restriction digest and DNA degradation - (reply: 3)
  410. what's wrong with my digestion - (reply: 2)
  411. Mystery band after vector digestion - (reply: 3)
  412. digestion with two REs requiring different buffer - (reply: 10)
  413. Restriction analysis of a vector - (reply: 17)
  414. Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
  415. Restriction Enzyme BstBI - (reply: 2)
  416. Problem with restriction digestion - (reply: 10)
  417. PCR - RFLP of plant cpDNA - Can I use several restriction enzyms together? (reply: 2)
  418. How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
  419. restriction digestion - (reply: 2)
  420. T4 blunting follow by digestion - (reply: 2)
  421. Question on digestion - (reply: 4)
  422. digestion with pvu1 enzyme - (reply: 3)
  423. genomic DNA PCR - RE digestion of gDNA before amplification (reply: 3)
  424. Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
  425. Restriction enzyme SBFI failed to cut plasmid - (reply: 3)
  426. methylation sensitive restriction site - (reply: 2)
  427. double digestion - Time for digestion (reply: 1)
  428. no restriction EcoRI in genomic DNA - (reply: 2)
  429. Double digestion/ligation problem - (reply: 4)
  430. restriction enzyme digestion problem - (reply: 7)
  431. Can I directly digest PCR products? - (reply: 9)
  432. Restriction Digest Woes - (reply: 1)
  433. Question about NcoI digestion... - (reply: 3)
  434. double digest not working? - BamHI+SacI double digest (reply: 4)
  435. unique restriction sites - (reply: 2)
  436. Double digestion of PCR product and ligation - (reply: 5)
  437. what's wrong? Plasmid digestion produce only smear - (reply: 6)
  438. Dnase I digestion - (reply: 3)
  439. Plasmid digestion - Problem with digestion (reply: 3)
  440. Question about activity of restriction enzymes - (reply: 3)
  441. Double digestion problem - (reply: 1)
  442. Enterokinase cleavage problem - HisTag (reply: 1)
  443. restriction map of seq alignment - (reply: 3)
  444. troubleshooting Rsa-I restriction digests - (reply: 2)
  445. restriction of pbluescript - restriction of pbluescript (reply: 4)
  446. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  447. restriction enzyme inactivation - How do you inactivate restriction enzymes? (reply: 4)
  448. digestion problem - incomplete digestion of transformed plasmid (reply: 1)
  449. problem with generation of restriction digestion site - (reply: 4)
  450. restriction enzyme mapping - (reply: 1)
  451. double restriction digestion - no ligation (reply: 6)
  452. Digest, refill an digestion - How can I do it? (reply: 2)
  453. Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
  454. Restriction site search - (reply: 7)
  455. maximum [EDTA] in restriction digests - (reply: 2)
  456. tRNA and digestion - (reply: 1)
  457. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
  458. Problems with restriction enzymes - (reply: 4)
  459. restriction mapping pDNA software - (reply: 1)
  460. Double Digestion - double digestion for southern (reply: 1)
  461. double digestion - double digestion (reply: 2)
  462. in- gel trypsin digestion - (reply: 1)
  463. DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
  464. digestion problems - (reply: 1)
  465. Adaptor for salI-EcoRI - Adaptor compatible for SalI and EcoRI (reply: 2)
  466. restriction enzime cutting of genomic DNA - (reply: 1)
  467. EcoRI cloning - correct orientation - Preferential orientation? (reply: 3)
  468. Dodgy Digest - (reply: 3)
  469. double digestion problem - (reply: 9)
  470. Ligate two restriction fragments of dsDNA - (reply: 6)
  471. Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
  472. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
  473. problems purifying plasmid DNA after restriction digestion - (reply: 3)
  474. software to find restriction site and TM for primer - (reply: 7)
  475. Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
  476. Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
  477. DNA precipitated out in digest reaction - (reply: 1)
  478. Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
  479. BamH1 and Xho1 double digestion problem - digestion problem (reply: 7)
  480. Regarding smaI restriction enzyme - Blunt end cloning (reply: 5)
  481. Genomic DNA Complete Digestion - (reply: 4)
  482. digest problem - extra bands (reply: 2)
  483. Enzyme digestion before bisulfite treatment - (reply: 1)
  484. DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
  485. PCR Primer Design with restriction sites - (reply: 1)
  486. CViJI restriction enzyme? - (reply: 2)
  487. Double digest with EcoR1 - would it work? - (reply: 1)
  488. partial digestion - match partial digestion conditions (reply: 1)
  489. Online tool for restriction digestion of DNA - (reply: 5)
  490. BamH1 and Nde1 double digestion problem - (reply: 3)
  491. After restriction digestion my bands just disappear - (reply: 3)
  492. restriction digestion - (reply: 2)
  493. A Digestion problem - (reply: 6)
  494. Restriction enzymes cut reaction - (reply: 1)
  495. plasmid restriction map- free software - (reply: 3)
  496. Double Digest problems - (reply: 2)
  497. frameshift after ligation - point-mutation in restriction site (reply: 3)
  498. Digestion time and enzyme - (reply: 2)
  499. digestion & purification problem - (reply: 6)
  500. Proteinase digestion of tissue for DNA extraction - (reply: 3)
  501. restriction digestion - plant genomic DNA (reply: 2)
  502. PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
  503. genomic DNA digest - (reply: 3)
  504. Restriction Digest - NarI-my very special friend! (reply: 4)
  505. Methylation and restriction digestion problem - (reply: 8)
  506. Double digestion and ligation problem - (reply: 15)
  507. Phages - How to digest lambda DNA (reply: 1)
  508. Restriction Digestion - problems (reply: 4)
  509. Restriction sites - (reply: 3)
  510. Restriction digestion problem!! - XbaI digestion (reply: 1)
  511. Does anyone the restriction map pEBG or pEBB - please help (reply: 2)
  512. star activity about BamH I and EcoR I - star activity about BamH I and EcoR I (reply: 1)
  513. Restriction digest - (reply: 1)
  514. DNA digestion time? - how long how many units (reply: 1)
  515. Protein Glycosylation/Thrombin cleavage of  GST fusion prote - Proteomics (reply: 2)
  516. Having trouble cutting pET19b with Nde I and BamHI - (reply: 4)
  517. Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
  518. enzymatic digestion - (reply: 3)
  519. Restriction sites - (reply: 2)
  520. Cant digest my vector - Enzyme digest failing (reply: 5)
  521. Difficult double digestion - (reply: 3)
  522. Restriction Mapping Problem - (reply: 1)
  523. restriction reaction - (reply: 2)
  524. EcoRI adaptor - (reply: 1)
  525. PCR restriction site - (reply: 2)
  526. The detailed digestion site map of pCO1 or pHaMDR1/A - (reply: 1)