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Restriction enzyme Related Discussions
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detection of restriction fragments - restriction fragments (reply: 1)
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Tool to investigate cleavage sites within a protein - (reply: 2)
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BamHI/XhoI ligation - (reply: 3)
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Double Digestion Restriction Enzyme - Troubles with double digestion (reply: 3)
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cloning vector and restriction mapping - (reply: 2)
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restriction enzyme digestion - (reply: 5)
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Double restriction digestion - (reply: 13)
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Restriction Mapping - Need help in clearing concept (reply: 1)
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Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
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Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
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RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
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restriction enzymes that cut at methylated cytosines - (reply: 2)
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DNA enzyme digestion problems - Smeared and stuck in wells, no or faint bands (reply: 1)
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dam methylation? - digestion problem (reply: 3)
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genomic DNA digestion - EcoRI+MseI - (reply: 2)
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double digestion time - (reply: 1)
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deisgning primers with restriction site - (reply: 7)
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Sequential digestion protocol - (reply: 3)
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precipitation during TEV cleavage - (reply: 1)
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Pulse Field Gel Electrophoresis (PFGE) - Secondary Restriction Enzyme Problem (reply: 2)
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In frame clonning adding a restriction site - (reply: 1)
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genomic library - use of restriction enzymes (reply: 10)
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Restriction enzymes not cutting - (reply: 4)
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Restriction Enzyme digestion - (reply: 4)
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Mysterious Band in NdeI/BamHI Double Digest - (reply: 7)
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failed restriction digests - (reply: 11)
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digestion at 4 degrees - (reply: 6)
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killer digest issues - (reply: 8)
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Xho1/Bglll digest of pSUPER.retro.puro with stuffer problems - (reply: 1)
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Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
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Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
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chromatin DNA and restriction endonucleases - (reply: 1)
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Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
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Why the addition of restriction sites to primers? - (reply: 3)
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No band is visible with BamHI and XbaI Digestion - (reply: 3)
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restriction enzyme sites - disruption (reply: 4)
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Digest with BglII and SalI - (reply: 1)
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Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
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plasmid digestion gel - (reply: 4)
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protein precipitation after enterokinase cleavage - (reply: 1)
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restriction digestion of plasmid - (reply: 8)
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Two restriction sites are close each other - Can I use them?? (reply: 3)
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Genome Restriction Digestion - (reply: 2)
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Digest Buffer Precipitation - Na2HPO4, L-Cysteine, EDTA (reply: 4)
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Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
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Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
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proteolytic cleavage - proteolytic cleavage (reply: 1)
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M13 Hind and Sal digest - (reply: 2)
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incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
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Smart Sofatware for Restriction Analysis - multiple simultaneous restriction analysis... (reply: 10)
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lux gene cloning using Sal I as restriction enzyme - (reply: 1)
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Sequencing incorrect after digestion and ligation - (reply: 5)
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Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
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Tool for complementary restriction enzymes? - REs producing overhangs to religate (reply: 5)
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digestion of pcr - (reply: 2)
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double digestion and ligation - (reply: 3)
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Restriction Enzyme Troubleshooting Help? - (reply: 2)
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Help restriction analysis of plasmid - (reply: 11)
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promoter pcr using primers with restriction sites giving trouble - (reply: 7)
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Aniline cleavage of RNA - (reply: 1)
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Thrombin digestion for GST - ppt problem (reply: 3)
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Multimerization after restriction digestion - (reply: 9)
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restriction enzyme digestion problem - (reply: 8)
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Help, sequestial digest - (reply: 1)
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help. Is this double digest right? - (reply: 5)
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HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
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Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
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Double digest and 3' end polishing with klenow - (reply: 1)
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Mystery band after Phenol:Chloro:IAA Extraction after restriction digest - (reply: 3)
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methylated restriction sites - (reply: 1)
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caspase-3 cleavage assay - how to test the clevage of a protein by caspase in vitro (reply: 2)
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how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
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Why does insert has two bands after RE digestion? - (reply: 3)
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No band after Double digestion - (reply: 4)
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PGEM-T easy gel - partial digestion? - (reply: 2)
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Primer design: how do I add a restriction site to my primer? - (reply: 2)
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Miniprep DNA digestion smear - (reply: 15)
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restriction enzyme - can i cut the noncoding region? - (reply: 2)
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how to insert a thrombin cleavage site - ....into my vector (reply: 3)
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only one band after double enzyme digestion - (reply: 4)
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Restriction Enzyme-EcoR V come frozen !? - (reply: 6)
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How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
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all the DNA gone after digestion - (reply: 8)
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EcoR1-Pst1 digest for vector and insert - (reply: 3)
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Vector digestion - (reply: 6)
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how to do double digestion with EcoRI and BamHI - (reply: 9)
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How to get rid the restriction sites in a plasmid ? - (reply: 5)
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NEB restriction enzyme BssS1 - digest (reply: 1)
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thrombin cleavage buffer - (reply: 1)
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General question about restriction sequences in PCR primers - (reply: 3)
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no band after digestion - (reply: 5)
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BamH1-Pst1 digest - (reply: 5)
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problems with restriction digestion - (reply: 7)
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how to add bases to the primer restriction sites - (reply: 2)
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southern blot - southern blot- restriction of genomic DNA (reply: 2)
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problem with restriction digest - I have an insert, but no vector.... (reply: 5)
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Restriciton digest protocol for adjacent sites? - (reply: 7)
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using restriction enzymes for studying DNA methylation - hemimethylation (reply: 3)
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Ecor1/Pst1 digest - (reply: 4)
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For double sequential digest: which enzyme first? - (reply: 7)
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Problems with double digestion - (reply: 1)
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restriction enzyme site with primers - (reply: 4)
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Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
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Extremely Low Yield PCR with restriction site addition - Assistance requested for beginning molecular biologist on PCR techniqu (reply: 3)
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can hyaluronidase be used to detach/digest cells? - (reply: 1)
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Dnase digestion after in vitro transcription - (reply: 4)
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How important is Ca2+ to thrombin cleavage? - (reply: 2)
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DNA smears after digestion - DNA smears after digestion (reply: 6)
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RE digestion Help needed everyone - (reply: 6)
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P/C/I extraction of plasmid after digestion - (reply: 1)
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double digestion of transformed bacteria colony - (reply: 7)
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4 enzyme digest and 3 piece ligation? - (reply: 3)
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Amt of restriction enzyme - (reply: 4)
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Does trypsin digest integral plasma membrane proteins? - (reply: 2)
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Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
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how to check if restriction digestion has happened - (reply: 7)
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Restriction enzyme sites mutate after ligation? - (reply: 2)
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Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
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Digestion dilemma - (reply: 5)
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cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
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Cloning PCR product blunt + restriction site - (reply: 2)
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Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
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Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
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smearing in the restriction digested products - (reply: 2)
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DNA degradation while digestion. - (reply: 3)
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HELP in Restriction Enzyme Digestion - troubleshooting re digestion (reply: 3)
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Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
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Proteases and thier site cleavage - Enzyme digestion (reply: 2)
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Problem with restriction enzyme NdeI - How many bases do I need? (reply: 6)
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Digestion question - (reply: 1)
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Integrity of overhang after digestion - (reply: 3)
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PstI/SapI digestion problem - restriction enzyme (reply: 3)
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How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
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function of restriction enzymme - (reply: 2)
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Double restriction enzyme digest - (reply: 3)
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NheI, EcoRI vector double digest - Troubling getting second enzyme to cut (reply: 6)
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Is this clone the correct one? - Restriction digestion doubt (reply: 1)
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SacII digestion - (reply: 1)
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how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
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Restriction Enzyme Binding - (reply: 3)
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NotI / EcoRI digest - (reply: 5)
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Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
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DNA loss in digestion - I lose lot of DNA in digestion (reply: 10)
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pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
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Problems with restriction enzyme digest. - (reply: 2)
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lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
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Restriction sites too close? - (reply: 2)
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Restriction digested plasmid - (reply: 2)
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Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
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Closing up restriction sites - KpnI/XhoI site needs to get closed (reply: 2)
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Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
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DNase Digestion and Reverse Transcription - (reply: 10)
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Restriction Fragment (urgent) - (reply: 4)
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Double digestion - (reply: 13)
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double digestion - (reply: 6)
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unable to digest my recombinant plasmid! - (reply: 9)
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Double Digestion with Single Restriction Enzyme - (reply: 2)
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plasmid DNA Digestion - (reply: 5)
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NsiI digestion problems - NsiI is not working properly? (reply: 1)
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Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
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problem with restriction digestion - (reply: 3)
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restriction digestion - (reply: 5)
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PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
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Introduce restriction sites with PCR primers - (reply: 2)
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Restriction enzyme digestion problem - (reply: 4)
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Concerning digestion enzymes - How do digestion enzymes work really? (reply: 1)
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Why should it be digested with a restriction enzyme before treated with bisulfi - (reply: 5)
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partial digestion of Tth111I - difficult??? (reply: 2)
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Where exactly does a restriction enzyme cut in its recognition site? - (reply: 1)
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Restriction of pichia vector pPICZ alpha with Xho1 and Xba1 - (reply: 1)
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Protein cleavage site? - (reply: 1)
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How can I delete a fragment that is not surrounded by restriction sites? - (reply: 3)
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partial digestion of Tth111I - (reply: 1)
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Question about Restriction enzyme - (reply: 3)
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Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
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digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
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Problem with Digestion or Ligation. How to tell? - (reply: 7)
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urgent HincII digestion problem - (reply: 5)
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Cloning help - how to break down a restriction site! (reply: 6)
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Digestion in sub-optimal buffer - (reply: 5)
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Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
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Double RE digest: using two different companies - (reply: 3)
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Double digest question for ligation - (reply: 4)
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Restriction mapping - (reply: 2)
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Restriction fragment length polymorphism - (reply: 1)
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Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
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Do restriction enzymes work on dUTP? - (reply: 1)
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Destruction of restriction site - (reply: 1)
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Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
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Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
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Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
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Protecting against Bgl II digestion - (reply: 2)
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Restriction digestion - (reply: 2)
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restriction enzymes primers - (reply: 5)
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Kpn1 restriction enzyme - (reply: 12)
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Sequential digestion - (reply: 3)
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What is wrong with my EcoRI digestion? - Can it attribute to the star activity? (reply: 6)
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Can restriction digestion be replaced by CHIP? - (reply: 5)
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Restriction digest of pQE plasmid - Double digestion problem (reply: 7)
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Digestion, Ligation or transformation problem? - (reply: 8)
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double digestion of plasmid - problem with incomplete digestion (reply: 9)
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ChIP- any experiences in using restriction enzymes instead of sonication? - (reply: 4)
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Nla III digestion - (reply: 2)
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Protein purification after cleavage (thioredoxin) - (reply: 3)
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is apoptosis always parp cleavage dependent? - cancer-parp-zvad-flow cyto (reply: 2)
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Vector with many restriction site - (reply: 6)
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DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
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Inhibition of BamHI - (reply: 4)
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cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
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Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
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enygmatic restriction by BamH1 - (reply: 8)
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Phusion kit altering my restriction sites only - (reply: 5)
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Restriction with two incompatible enzymes - (reply: 4)
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Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
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Restriction site - (reply: 3)
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double digestion with BamHI and HindIII - (reply: 13)
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Anyone used restriction enzyme BsaI? - (reply: 3)
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How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
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RNAse and DNA digestion in Southern Blot - (reply: 3)
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I need help with the steps in sequential digests please - (reply: 3)
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BAC DNA restriction analysis - I need help :( (reply: 4)
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restriction map - (reply: 7)
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Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
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non-specific cleavage products in an endonuclease assay - (reply: 3)
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How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
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Can any one help me with restriction digestion map for DNA? - (reply: 3)
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Is a Triple Restriction Digest Possible? - (reply: 6)
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Clonning with one restriction enzyme - Some help please (reply: 2)
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Restriction digestion of PCR product - (reply: 3)
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Restriction enzyme specificity for thymine and DNA - are thymine/uracil and DNA/RNA interchangeable? (reply: 1)
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AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
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Is restriction enzyme Srf1 a good cutter? - (reply: 4)
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Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
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Problem on restriction digest - Inquire (reply: 11)
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ppt DNA directly from a digestion/ pcr - (reply: 2)
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Restriction digest - volume of restriction digest (reply: 2)
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RE digest and klenow - sequential blunting (reply: 6)
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Help with AVRII digestion - (reply: 1)
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Restriction enzyme -Eco47III - (reply: 1)
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his-tag thrombin cleavage in-column? - as a purification strategy (reply: 1)
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which restriction enzyme should I use to digest the vector? - (reply: 3)
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forgot to digest PCR insert before ligation. - what happened? (reply: 7)
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restriction site in primers urgent - (reply: 1)
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Methylated DNA Interfering with Restriction Digestion - Restriction digestion problems (reply: 3)
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Is my restriction digest not cutting properly? - (reply: 8)
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Restriction site clonning failed - (reply: 9)
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Primer design with restriction sites - (reply: 3)
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can I take some of ligation mix and digest? - (reply: 4)
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Restriction Enzyme and Sequencing Problems - (reply: 1)
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restriction enzymes - (reply: 4)
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PCR band for water but no contamination, Digestion not working - (reply: 10)
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digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
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After double digest my plasmid is 2Kbp shorter! - (reply: 3)
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double restriction enzyme digestion - not work? (reply: 6)
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gfp digested with Ecor1 + Hind III, and Bgl II - (reply: 4)
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Restriction digestion - (reply: 7)
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My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
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3 bands after digestion - (reply: 13)
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thrombin cleavage and triton? - protein purification (reply: 2)
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Southern Blot: which restriction enzyme? - (reply: 1)
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Hepg2 cells resistant to trypsin digestion - (reply: 7)
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Restriction digestion by SpeI and SphI - (reply: 2)
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PacI digestion - (reply: 3)
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please tell me more about primers with restriction site! - (reply: 4)
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Restriction sites frequencies in mouse genome - (reply: 10)
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restriction of Open Reading Frame (ORF) - (reply: 1)
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Few colonies with cloning of PCR product after digestion - (reply: 7)
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TM for primers with restriction secuence tail. - Suggestion (reply: 3)
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digesting out restriction site - (reply: 6)
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Can DNA ethanol precipitation remove restriction enzyme? - (reply: 7)
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Religation with single digest vector - (reply: 7)
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Identify insert by restriction digestion - (reply: 6)
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Afl II linearization & ligation - (reply: 3)
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Problems with SalI digestion - (reply: 5)
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How to add restriction enzyme sites to the primer - (reply: 6)
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PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
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pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
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is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
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restriction direct to ligation? - skip clean up? (reply: 12)
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Do I have to purify vector after RE digestion for cloning? - (reply: 17)
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Cleavage of target protein from glutathione sepharose - (reply: 3)
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Tryptic digest help - Trouble diffusing stain (reply: 3)
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Factor Xa Cleavage - MBP-Factor Xa Cleavage (reply: 2)
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Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
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Problem with DNase digestion of RNA - (reply: 6)
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RE digest and subsequent ligation - A base is now missing from restriction site - how, why? (reply: 7)
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Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
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trouble with restriction digestion - (reply: 3)
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adding restriction sites to degenerate primers - (reply: 9)
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BAC digestion - (reply: 8)
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Restriction digest as a template - (reply: 2)
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restriction to ligation - (reply: 5)
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Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
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Problem with BamHI/SalI double digestion - (reply: 8)
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disappearing vector after digestion - (reply: 11)
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restriction digestion of PCR product? - (reply: 10)
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Removing vector restriction sites - and replacing with new ones (reply: 8)
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Stuborn band after digestion of plasmid DNA - (reply: 4)
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double digestion problem - additional band after double digestion (reply: 2)
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single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
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NdeI double digestion - (reply: 3)
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How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
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Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
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Restriction buffer - (reply: 3)
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problems with NdeI restriction enzyme? - (reply: 2)
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is there a restriction about amount of vector in a ligation? - (reply: 14)
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Full and Partial Digestion - (reply: 3)
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smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
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Basic Question Restriction Digest - (reply: 11)
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ApaI / Not I double digestion problem - (reply: 6)
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XbaI/BamHI double digest- dam methylation detection problem - (reply: 3)
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Digestion Problem with BsmBI - (reply: 3)
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Vortexing restriction digests? - (reply: 5)
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If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
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Cloning a PCR product after digestion - (reply: 7)
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Question about proteinase cleavage sites - (reply: 1)
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dimers of dna (not primers) following digest or pcr? - (reply: 11)
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KpnI and BamHI double digest help - (reply: 6)
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cloning restriction fragment for sequencing - (reply: 1)
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reverse primer design with restriction site - (reply: 6)
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Pronase digestion - (reply: 1)
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stability of protein sample after in-gel digestion - (reply: 2)
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Would ExoI work? - Alternative nuclease for DNA digestion (reply: 1)
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QIAquick PCR for preparing for restriction digestion - very low concentrations (reply: 4)
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Unique restriction sites in two sequences - (reply: 3)
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How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
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peptidase cleavage sites - (reply: 3)
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problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
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website for identifying restriction sites in a gene - (reply: 6)
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southern and restriction enzyme - what enzyme to choose (reply: 6)
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sequencing a restriction fragment - (reply: 4)
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Restriction Analysis - (reply: 1)
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keep getting incomplete digest for plasmid - (reply: 3)
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Insertions/deletions at cloning restriction site - (reply: 1)
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double digest pcDNA(+) by XhoI&XbaI, uncompletely? - (reply: 3)
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CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
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How to fill in 5` overhangs after Restriction digestion - (reply: 2)
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Effect of bile concentration on human digestion - Human digestion (reply: 5)
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Role of BSA in restriction enzyme digestion - help (reply: 2)
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restriction digestion problems - digestion problems with SalI (reply: 2)
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Reagents used...in restriction digestion and electrophoresis - (reply: 2)
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protein digestion - (reply: 1)
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restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
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trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
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RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
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do you sonicate or digest with lysozyme? - which one do you prefer and why.... (reply: 21)
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Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
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How does normal mRNA escape from cleavage by dicer? - (reply: 5)
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Is A-tailing of restriction fragment possible? - (reply: 2)
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Lipase enzyme digestion of milk experiment - effects of stale milk - Help needed understanding how stale milk could affect my experiment... (reply: 2)
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Help on digestion and ligation...colonies not wanted why? - (reply: 4)
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problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
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How to extract a 20kb vector after double digestion - (reply: 4)
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double digestion and dephosphorylation - (reply: 5)
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His tag cleavage by Thrombin - (reply: 2)
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Restriction digest - (reply: 6)
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Restriction enzyme that cut CGCGCG? - (reply: 3)
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Interpreting RE digest results after blunt cloning - (reply: 1)
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No band on double digestion of construct - (reply: 1)
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does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
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BclI digestion - (reply: 4)
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Digestion Problem - Cloning (reply: 5)
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basic RE digests acting weird - (reply: 3)
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Nhe1 Restriction digestion - (reply: 3)
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Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
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tricky digestion for cloning gurus - (reply: 13)
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RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
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EcoRI problem - (reply: 4)
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partial digestion problem - (reply: 8)
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Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
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my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
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Cloning - RE digestion clean up (reply: 2)
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do primers and dNTPs alter digestion efficiency? - (reply: 4)
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Enzyme Restriction - (reply: 4)
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Incomplete genomic digestion for Southern - (reply: 1)
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Unexplained His-tag cleavage - (reply: 2)
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pQE30 sequence needed - or restriction map (reply: 3)
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Plasmid appears as two bands after double digestion - (reply: 2)
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attach adaptors or PCR the restriction enzyme sites? - which one would you suggest and why (reply: 2)
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ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
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Partial digest to destroy an enzyme site - (reply: 10)
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CIP with restriction digestion - (reply: 5)
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to purify these PCR products or not? - preparation for restriction digest (reply: 5)
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Use EcoRI/KpnI, good combination? - (reply: 4)
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HindIII digestion - (reply: 1)
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how many bases are needed upstream of restriction site? - (reply: 5)
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Restriction with pstI - (reply: 1)
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restriction enzyme specificity - (reply: 7)
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Bisulfite conversion - necessary to digest DNA? - (reply: 1)
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BamHI/XbaI cloning, bad combination? - (reply: 3)
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GST Tag Cleavage - (reply: 2)
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how do you inactivate the enzymes after digestion? - (reply: 17)
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PCR primer: 2 restriction sites? - (reply: 4)
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Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
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HELP! selective scission - thrombin digestion (reply: 1)
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gel purification after restriction digestion - (reply: 3)
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Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
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How 3M KAc to influence restriction enzyme activity? - (reply: 4)
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Restriction map of pUC18 - (reply: 2)
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why we use BVS in DNA digestion? - (reply: 3)
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Retroviral Vector won't digest ideas! - (reply: 1)
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restriction digestion problem... - (reply: 12)
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unpredictable plasmid - can digest but not sequence. . . (reply: 4)
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Problem with BsaB1 digestion - (reply: 1)
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how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
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Southern - Southern Blot Digestion (reply: 4)
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how do you check your restriction enzyme is working? - (reply: 12)
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Role of restriction and TAE Buffer - (reply: 1)
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restriction digest and DNA degradation - (reply: 3)
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what's wrong with my digestion - (reply: 2)
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Mystery band after vector digestion - (reply: 3)
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digestion with two REs requiring different buffer - (reply: 10)
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Restriction analysis of a vector - (reply: 17)
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Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
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Restriction Enzyme BstBI - (reply: 2)
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Problem with restriction digestion - (reply: 10)
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PCR - RFLP of plant cpDNA - Can I use several restriction enzyms together? (reply: 2)
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How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
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restriction digestion - (reply: 2)
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T4 blunting follow by digestion - (reply: 2)
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Question on digestion - (reply: 4)
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digestion with pvu1 enzyme - (reply: 3)
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genomic DNA PCR - RE digestion of gDNA before amplification (reply: 3)
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Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
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Restriction enzyme SBFI failed to cut plasmid - (reply: 3)
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methylation sensitive restriction site - (reply: 2)
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double digestion - Time for digestion (reply: 1)
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no restriction EcoRI in genomic DNA - (reply: 2)
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Double digestion/ligation problem - (reply: 4)
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restriction enzyme digestion problem - (reply: 7)
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Can I directly digest PCR products? - (reply: 9)
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Restriction Digest Woes - (reply: 1)
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Question about NcoI digestion... - (reply: 3)
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double digest not working? - BamHI+SacI double digest (reply: 4)
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unique restriction sites - (reply: 2)
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Double digestion of PCR product and ligation - (reply: 5)
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what's wrong? Plasmid digestion produce only smear - (reply: 6)
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Dnase I digestion - (reply: 3)
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Plasmid digestion - Problem with digestion (reply: 3)
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Question about activity of restriction enzymes - (reply: 3)
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Double digestion problem - (reply: 1)
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Enterokinase cleavage problem - HisTag (reply: 1)
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restriction map of seq alignment - (reply: 3)
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troubleshooting Rsa-I restriction digests - (reply: 2)
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restriction of pbluescript - restriction of pbluescript (reply: 4)
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Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
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restriction enzyme inactivation - How do you inactivate restriction enzymes? (reply: 4)
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digestion problem - incomplete digestion of transformed plasmid (reply: 1)
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problem with generation of restriction digestion site - (reply: 4)
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restriction enzyme mapping - (reply: 1)
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double restriction digestion - no ligation (reply: 6)
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Digest, refill an digestion - How can I do it? (reply: 2)
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Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
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Restriction site search - (reply: 7)
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maximum [EDTA] in restriction digests - (reply: 2)
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tRNA and digestion - (reply: 1)
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cloning genes with incompatible restriction sites - Protein expression (reply: 2)
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Problems with restriction enzymes - (reply: 4)
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restriction mapping pDNA software - (reply: 1)
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Double Digestion - double digestion for southern (reply: 1)
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double digestion - double digestion (reply: 2)
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in- gel trypsin digestion - (reply: 1)
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DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
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digestion problems - (reply: 1)
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Adaptor for salI-EcoRI - Adaptor compatible for SalI and EcoRI (reply: 2)
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restriction enzime cutting of genomic DNA - (reply: 1)
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EcoRI cloning - correct orientation - Preferential orientation? (reply: 3)
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Dodgy Digest - (reply: 3)
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double digestion problem - (reply: 9)
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Ligate two restriction fragments of dsDNA - (reply: 6)
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Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
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Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
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problems purifying plasmid DNA after restriction digestion - (reply: 3)
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software to find restriction site and TM for primer - (reply: 7)
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Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
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Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
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DNA precipitated out in digest reaction - (reply: 1)
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Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
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BamH1 and Xho1 double digestion problem - digestion problem (reply: 7)
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Regarding smaI restriction enzyme - Blunt end cloning (reply: 5)
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Genomic DNA Complete Digestion - (reply: 4)
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digest problem - extra bands (reply: 2)
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Enzyme digestion before bisulfite treatment - (reply: 1)
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DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
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PCR Primer Design with restriction sites - (reply: 1)
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CViJI restriction enzyme? - (reply: 2)
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Double digest with EcoR1 - would it work? - (reply: 1)
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partial digestion - match partial digestion conditions (reply: 1)
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Online tool for restriction digestion of DNA - (reply: 5)
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BamH1 and Nde1 double digestion problem - (reply: 3)
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After restriction digestion my bands just disappear - (reply: 3)
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restriction digestion - (reply: 2)
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A Digestion problem - (reply: 6)
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Restriction enzymes cut reaction - (reply: 1)
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plasmid restriction map- free software - (reply: 3)
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Double Digest problems - (reply: 2)
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frameshift after ligation - point-mutation in restriction site (reply: 3)
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Digestion time and enzyme - (reply: 2)
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digestion & purification problem - (reply: 6)
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Proteinase digestion of tissue for DNA extraction - (reply: 3)
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restriction digestion - plant genomic DNA (reply: 2)
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PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
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genomic DNA digest - (reply: 3)
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Restriction Digest - NarI-my very special friend! (reply: 4)
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Methylation and restriction digestion problem - (reply: 8)
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Double digestion and ligation problem - (reply: 15)
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Phages - How to digest lambda DNA (reply: 1)
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Restriction Digestion - problems (reply: 4)
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Restriction sites - (reply: 3)
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Restriction digestion problem!! - XbaI digestion (reply: 1)
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Does anyone the restriction map pEBG or pEBB - please help (reply: 2)
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star activity about BamH I and EcoR I - star activity about BamH I and EcoR I (reply: 1)
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Restriction digest - (reply: 1)
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DNA digestion time? - how long how many units (reply: 1)
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Protein Glycosylation/Thrombin cleavage of GST fusion prote - Proteomics (reply: 2)
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Having trouble cutting pET19b with Nde I and BamHI - (reply: 4)
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Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
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enzymatic digestion - (reply: 3)
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Restriction sites - (reply: 2)
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Cant digest my vector - Enzyme digest failing (reply: 5)
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Difficult double digestion - (reply: 3)
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Restriction Mapping Problem - (reply: 1)
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restriction reaction - (reply: 2)
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EcoRI adaptor - (reply: 1)
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PCR restriction site - (reply: 2)
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The detailed digestion site map of pCO1 or pHaMDR1/A - (reply: 1)