Could anyone help me with Realtime-PCR? - (Jun/23/2005 )
I want to do a Realtime-PCR in botany. But i don't know which gene is the best reference gene whose expression is stable in root, leaf ,caudex and other tissue.
Could anyone give me some adviese on it? Thank you very much
Sean
-PCR_Fudan-
QUOTE (PCR_Fudan @ Jun 23 2005, 03:02 AM)
I want to do a Realtime-PCR in botany. But i don't know which gene is the best reference gene whose expression is stable in root, leaf ,caudex and other tissue.
Could anyone give me some adviese on it? Thank you very much
Sean
Could anyone give me some adviese on it? Thank you very much
Sean
Hi,
Try to look at DNA polymerase gene and ribosomal DNa gene.
All these genes are housekeeping gene which will presence in all tissue.
regards
Hadrian.
-Hadrian-