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Help a clinician - Calprotectin (Mar/09/2005 )

Greetings,
I'm a paediatric gastroenterologist and starting PhD research into inflammatory states affecting children and a specific interest into Calprotectin and its application as a marker of inflammation in the gut (inflammatory bowel disease, gut permeability, food allergies, etc.).

'Purely' medical journals explain basic information on the validity of this molecule as an aid to test the presence or absence of disease in the gut.

However since I am a clinician, it is rather hard to find comprehensive information about this molecule's structure, its origins, its specific inter/actions and effects. I would be very grateful indeed if someone could point me in the right direction. Non-clinical literature searches have yielded alot of very detailed information that is confusing at best! Does a map of pathway/s exist? Is there just one chemical name for this molecule or do more exist?

I would be very very very happy indeed to read from anyone who could help me in this endeavour.

Many thanks,

Konrad

-Konrad-

QUOTE (Konrad @ Mar 9 2005, 11:54 AM)
Greetings,
I'm a paediatric gastroenterologist and starting PhD research into inflammatory states affecting children and a specific interest into Calprotectin and its application as a marker of inflammation in the gut (inflammatory bowel disease, gut permeability, food allergies, etc.).

'Purely' medical journals explain basic information on the validity of this molecule as an aid to test the presence or absence of disease in the gut.

However since I am a clinician, it is rather hard to find comprehensive information about this molecule's structure, its origins, its specific inter/actions and effects. I would be very grateful indeed if someone could point me in the right direction. Non-clinical literature searches have yielded alot of very detailed information that is confusing at best! Does a map of pathway/s exist? Is there just one chemical name for this molecule or do more exist?

I would be very very very happy indeed to read from anyone who could help me in this endeavour.

Many thanks,

Konrad


have you tried places like drugbank or maybe even ensembl?

-Kami23-

QUOTE (Konrad @ Mar 9 2005, 03:54 AM)
... comprehensive information about this molecule's structure, its origins, its specific inter/actions and effects.
Konrad]


This Seems to be a GOOD REVIEW ARTICLE

Here are some papers that might interest you; you might try to find Dr.Burns' or Dr.Bisset's research pages for links and info and such:

Burns S, Bisset W, Golden B. Faecal calprotectin: an objective measure of bowel inflammation in childhood inflammatory bowel disease. J Paediatr Gastroenterol Nut2001; 32:171–7.

Bunn S, Bisset W, et al. Faecal calprotectin: validation as a non-invasive measure of bowel inflammation in childhood inflammatory bowel disease. J Paediatr Gastroenterol Nut2001; 33:14–22.

and this one,
A pretty accessible paper imho

QUOTE (Konrad @ Mar 9 2005, 03:54 AM)
... Does a map of pathway/s exist?
Konrad
To some extent: there have been studies which investigate the molecular signalling cascade, indicating a relationship to the CD-14–Toll-like receptor–nuclear factor κB pathway
[url="http://www3.interscience.wiley.com/journal/118871701/abstract?CRETRY=1&SRETRY=0"]See Here
and also that the cytotoxic effect is mediated by exclusion of zinc from cells See Here
(also check the references listed at the end of the paper).
Also, it has been shown that calprotectin "...induced cell death involves Bak, selective release of Smac/DIABLO and Omi/HtrA2 from mitochondria, and modulation of the balance between pro- and anti-apoptotic proteins."
See Here, but watch out for the conclusion about RAGE signalling,! as there are other studies that indicate RAGE signalling pathway is involved but not in apoptosis, rather in small dose calprotectin induced cell growth.Link Here


QUOTE (Konrad @ Mar 9 2005, 03:54 AM)
Is there just one chemical name for this molecule or do more exist?
Konrad


Calprotectin, also known as MRP8/MRP14 or S100A8/A9 see link here Also, check the 'relevant literature' section at the bottom for links to papers and resources.

Hope this was helpful!

-k_undertoe-

For almost any pathway go to:biocarta.com

-merlav-

Try Pubmed from the NIH (http://www.ncbi.nlm.nih.gov./sites/entrez?db=PubMed). They have a database of protien structures and one of the most comprehensive databases of literature I know of.

-gfischer-