The role of NP-40 vs Triton X-100 - (Jan/24/2005 )
I found a lot of lysis buffer are different in terms of non-denaturing reagents, such as NP-40 AND Triton X-100. But does both of them have different roles? I am wondering...
-Cindyflying-
According to the Sigma product info sheet:
QUOTE
Triton X-100tm has a structure very similar to those of Igepal CA-630 and of Nonidet P-40 (no longer commercially available), and the names are sometimes reported as synonyms. However, Triton X-100 is slightly more hydrophilic than Igepal CA-630; these two detergents are NOT considered to be functionally interchangeable in most applications.
In my hands, NP40 is more stringent than TX100 when used at the same percentages.
-badcell-
QUOTE (badcell @ Jan 27 2005, 01:02 PM)
In my hands, NP40 is more stringent than TX100 when used at the same percentages.
maybe np-40 is the equivalent of tx-114 (a purer fraction of tx-100)
-mdfenko-
hi,
perform an experiment with both detergents(NP-40 n tritonX100) using percentages range(0.1, 0.5 &1%) and see the lysis capacity by using same number of cells.
observe ur results, may be u will be the one who can answer ur question.
please let me know the results if u do this experiments.
gud luck
QUOTE (Cindyflying @ Jan 24 2005, 10:59 AM)
I found a lot of lysis buffer are different in terms of non-denaturing reagents, such as NP-40 AND Triton X-100. But does both of them have different roles? I am wondering...
-payeli-