to PCR the PCR product - (Jan/13/2009 )

Hi!!
If I'll do a PCR (using a cDNA pool) using two specific primers, then elute the PCR product, can I re-do the same PCR using the same primers?
I'll expect to have the product?
Thanks

---

It should work.

1. Nested primers work better and give more specific product. Without that you may end up with some non-specific products, but by all means you can try and see if you are lucky.

2. Don't expect that all products you will get will be specific in this case.

3. Sequencing of this product with the same primers will most likely give you dirty results. A sub-cloning step is recommendable.

---

Of course it works.!!! (Nested - pcr!! ) but perphaps you will get inespecific bands

---

Be carefull!! Use very little of PCR fragment you amplified, otherwise your reaction will be over too soon!!

---