PCR efficiency? - (Nov/07/2008 )

I have got problems with pcr efficiency. I´m not quite sure if the problem is that i have got miRNA enriched fractions instead of total rna or if the cDNA dilutions of the microRNA shouldn't be stored at 4° for longer than a few days > degradation problem or if it is just a pipet problem.
Anybody has experienced similar problems?
Thank you for any suggestion.

I'd suggest looking into PCRboost. Its a product I found that really helps my PCR reactions. Its from a company called Biomatrica. Not sure if it will help you with your problem, but at least look into it. It definitely helped me with mine!

Austin

Thanks for your reply. Maybe it wasn´t clear, but I use real time pcr, and I think the product shouldn´t be used for it, i found this in the product description: >PCRboost® is a reagent that enhances end-point or reverse transcription-PCR<. Anyway, I didn´t know something like that exists, so thank you for your information.

I've never stored my samples at 4oC, except when they are on ice for a short period of time. I always store them at -20 and it seems to be fine.