questions - plant sterile (Oct/08/2008 )

hi all


Now I come across with a problem about my study.

When I overexpress the gene which i am interested in and get the
transgenic plant.Then I find that some lines have the phenotype which is
sterile.

However I do not know why this overexpression can cause sterile. Then
I analyze the expression pattern of the promoter. But it does not
express neither in pollen nor pollen tube. It expresses in guard cells of
stoma and style.

According to the expression, I guess the gene may influence the style
and cause sterile. But I do another experiment. I pollinate the line
with other pollen and then it has the fertilities.

Could someone tell me some possibilites??

Thanks a lot

stone

you will mostly come across problems on your studies

before you continue doing more experiments i'd recommend you to look back and check all the information you have about your gene, what routes it is involved in, any function that could be affected indirectly to give the (apparently) facultative sterility.

good luck. see you around.

you will mostly come across problems on your studies

before you continue doing more experiments i'd recommend you to look back and check all the information you have about your gene, what routes it is involved in, any function that could be affected indirectly to give the (apparently) facultative sterility.

good luck. see you around.