calcium in PBS - how much? - (Sep/30/2008 )

we like to add calcium to PBS to prevent detaching of fixed cells; we plan to filter the precipitate;

how much calcium (chlorid) do you add? adding calcium phosphate is hopeless, isn´t it?

-The Bearer-

We buy the 10x DPBS (14080) with calcium and magnesium, from invitrogen for immunostaining to sprevent cells from detaching.

CaCl2 (invitrogen website) - Calcium Chloride (CaCl2) (anhyd.) Mw-111 conc.-1000mg/L
MgCL2- Magnesium Chloride (MgCl2-6H2O) Mw- 203, conc.- 1000mg/L

-scolix-

QUOTE (scolix @ Sep 30 2008, 06:05 AM)

thanks scolix, I searched for it; the D stands for Dulbecco to my surprise; I thought of a more inofficial way to treat PBS with Ca2+ and Mg2+

-The Bearer-