Mung Bean Nuclease - (Aug/26/2004 )

Hello!

Who from you used Mung-Bean-Nuclease for polishing Restriction-Sites (In my case its a 3'overhang)

What are/were your experiences? good/bad??

In our institute nobody seems ever to have used it..

greeetings!

yeah, mung bean nuclease works great most of the time, just keep to the manufacturers datasheet.

Only once I had no - read: absolutely no- DNA left after I forgot a MBN digest for over half a day...

mike