How to get rid of bubbles on ELISA plates? - (Dec/11/2007 )
Hi, all,
How do you guys get rid of bubbles on ELISA plates? Hot needle method is slow and there are too many of these bubbles. Thanks.
gh
Hi, genehunter. I use an small micropipette tip or something similar, blotting it in whatmann paper or similar when it lose "activity" (it must be dry to destroy bubbles). Unfortunately, it is not a quickly method, but that's how we do it in my lab...
hallo,
we use a commercial hair-dryer.
we use a commercial hair-dryer.
good one. I will try it. Thx.
Simple answer (but not necessarily helpful to you now): 
Reverse pipette your samples, so you don't make bubbles.
Bubbles in wash step don't matter, obviously...
Blower worked well. Thanks! 
swanny, I have some detergents in it and I was running a reaction using the ELISA plates, not just ELISA 
.
we use a commercial hair-dryer.
sword413:
what was that for? Are you trying to gain enough post number so that you can start to request papers or something?
We use a very quick flame of the plate, similar to removing bubbles from agar plates.
How do you guys get rid of bubbles on ELISA plates? Hot needle method is slow and there are too many of these bubbles. Thanks.
gh
there are several methods: f.i. short incubation under vacuum, or a hot needle