TUNEL Staining - non-fluorescent (Nov/29/2007 )

hallo,

1st Q: i want to change my TUNEL staining method (In situ Cell Detection Kit, Fluorescein). does anyone has experience with TUNEL stainings which i can check in light-microscope?

2nd Q: is it possible that macrophages and other leukocytes (infiltrates) are prone to false positive TUNEL stains in tissue sections?

thanks for supportive replies

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not sure we are looking for the same thing but i'd trust (cleaved) caspase 3 over tunel any day

dom

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I was told that TUNEL staining can give false positive results in general.

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Ideed. We used here in our lab to analyse T cells coincubated with tumor cells and the T cells were also tunel positive (not expected).
We think that maybe because the T cells are prone to recombination (T cell receptor), they have chromosome breaches and then
are also stained.

cheers

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