PMA dose dependent effect - (Sep/21/2007 )

Hi,

Does PMA have a dose dependent effect? I read an article in which 1 uM during 16h seem to have an inactivation of the PKC and in which 100 nm during 10 minutes activated the PKC. What is the mechanism?

Thanks a lot!

Brassap

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hah, this is nice myriads of papers of the past 30 years try to answer this and similar questions of TPA effects; or in other words: there is no simple explanation to your question;

at least, there should be discriminated between the PKC subfamilies and subfamily members; f.i. aPKC are not to activate by PMA which means other PKC isoforms of cPKC and nPKC are activated by TPA; there are other targets of PMA such as Munc13, chimaerins and others; an important point is also the cell type and involved first messengers

to get a clue think in terms of positive and negative feedback mechanisms for activation/deactivation of PKC and gene expression/repression

one thing appears to be clear: PMA has a concentration-dependent effect

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Thank you very much for your reply, this is a useful information for me!

Brassap

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