Glass Spectrophotometer Tubes ! - what's wrong with them (Aug/09/2007 )
QUOTE
quartz is uv transparent and good for both uv and visible applications. also, since it is used for cuvettes, will be manufactured to finer tolerances
mdfenko, what do u mean by 'finer tolerances' !? and is quartz=crystal!
QUOTE
lime glass is not recommended for use in a spectrophotometer but may be usable
Why?!
-strawberry-
QUOTE (strawberry @ Aug 23 2007, 05:01 AM)
QUOTE
quartz is uv transparent and good for both uv and visible applications. also, since it is used for cuvettes, will be manufactured to finer tolerances
mdfenko, what do u mean by 'finer tolerances' !? and is quartz=crystal!
QUOTE
lime glass is not recommended for use in a spectrophotometer but may be usable
Why?! finer tolerances refers to attributes such as clarity, regularity of wall thickness, foreign particles, etc.
quartz is a silicate crystal.
lime glass tubes are usually thicker walled than borosilicate and show a general green tinge.
-mdfenko-
what about the ability of getting reactions between the material (glass or quartz) and colors of solutions, particles,.......
i heard once that this may affect the final readings !!
-strawberry-
yes, it does happen. and it may affect the readings. i find that the bradford protein determination leaves a residue behind on the cuvette. we wash the cuvette with alcohol to remove it between all readings.
particulates will scatter the light and cause the appearance of absorbance.
-mdfenko-
thanx mdfenko , but which type 'stick' more particulates, glass or the quartz cuvettes?!
-strawberry-
QUOTE (strawberry @ Aug 26 2007, 04:59 AM)
thanx mdfenko , but which type 'stick' more particulates, glass or the quartz cuvettes?!
with the bradford method, it didn't appear to matter. both bound a lot of dye so we use disposable plastic for this procedure.
as for what will bind more, it depends on the solution but i think that glass may bind more, in general. you still have to clean any cuvette thoroughly.
-mdfenko-