LDH assay to get GI50? - (Jun/01/2007 )

I am a newbie here and my question might be a stupid one, but I am quite confused.

I am trying to get GI50 value for a test drug and can't use MTT or other cell viability assays to detect metabolically active cells.
I'd like to use LDH release assay that depends on LDH release from dead cells.
The question that I have is if I use LDH assay, I am detecting the number of dead cells. Then how can I get GI50 value out of the number of dead cells? Don't I need the number of live cells to get the growth inhibition??

Can anyone help me?

Thanks!

hi,

Why cant you use MTT?
there are other ways to measure proliferation or in your case inhibition of proliferation.
you can trypsinize or collect in any other way the cells and count them in every time point or concentration; or use incorporation of labeled-nucleotide; or measure glucose consumption...and more