band densities - (Mar/12/2007 )

Hi

I am looking for a free downlodable or online software for measuring western blot band densities. I have my files in TIFF & JPEG format from the X-Ray film.

Please suggest the software if you know any.

Thank you

-rudra09-

QUOTE (rudra09 @ Mar 12 2007, 05:17 PM)

We use TotalLab program. It works with TIFF format. But in net I think you can only download try&buy version.

-circlepoint-

ImageJ is free and very good:

http://www.protocol-online.org/forums/inde...1&hl=imagej

-Pumuki-

Hi

I downloaded the ImageJ program and tried my blot in TIFF format.

I first selected the band as select first lane and then other bands as selected the next lane.

I then used the gel analyser option and plot lanes which gave the U shaped graphs. Using wand (tracing tool) I tried to draw line but I am not sure how to do and how to get the values then.

Please advise how to do. If you have any picture which has wand tool in the histogram that will be real help for people who are trying to use this program.

Thank you

QUOTE (circlepoint @ Mar 12 2007, 11:12 PM)

QUOTE (rudra09 @ Mar 12 2007, 05:17 PM)

We use TotalLab program. It works with TIFF format. But in net I think you can only download try&buy version.

-rudra09-

http://www.protocol-online.org/forums/inde...showtopic=25469

-tryptofan-